Hydrogen inhalation alleviates nonalcoholic fatty liver disease in metabolic syndrome rats
Hydrogen inhalation alleviates nonalcoholic fatty liver disease in metabolic syndrome rats
复制标题
吸入氢气可减轻代谢综合征大鼠的非酒精性脂肪肝
DOI：
10.3892/mmr.2020.11364
复制
发表时间：
2020-10-01
影响因子：
3.4
通讯作者：
Qin, Shucun
中科院分区：
文献类型：
--
作者：
Liu, Boyan;Xue, Junli;Qin, Shucun
氢气对多种疾病具有治疗和预防作用。本研究探讨了吸氢对高脂肪和果糖饮食（HFFD）诱导的 Sprague-Dawley 大鼠非酒精性脂肪肝（NAFLD）的潜在保护作用和剂量依赖性方式。大鼠被随机分为四组：i）对照组，常规饮食/空气吸入； ii) 模型组，HFFD/空气吸入； iii) 低氢组，HFFD/4%氢气吸入； iv) 高氢组，HFFD/67%氢气吸入。经过 10 周的实验，吸氢改善了喂食 HFFD 的大鼠的体重增加、腹部脂肪指数、肝脏指数和体重指数，并降低了口服葡萄糖耐量试验中的曲线下总面积。吸入氢气还改善了肝脏脂质含量以及丙氨酸转氨酶和天冬氨酸转氨酶活性的增加。用苏木精和伊红以及油红O染色评估的肝脏组织病理学变化显示，吸氢组的脂质沉积较低，这与脂质合成基因SREBP-1c表达的减少一致。大多数指标在氢气处理后受到剂量依赖性的影响。总之，吸氢可能通过影响NAFLD代谢综合征大鼠模型的一般状态、脂代谢参数、肝组织学和肝功能指标而发挥保护作用。
Hydrogen exhibits therapeutic and preventive effects against various diseases. The present study investigated the potential protective effect and dose-dependent manner of hydrogen inhalation on high fat and fructose diet (HFFD)-induced nonalcoholic fatty liver disease (NAFLD) in Sprague-Dawley rats. Rats were randomly divided into four groups: i) Control group, regular diet/air inhalation; ii) model group, HFFD/air inhalation; iii) low hydrogen group, HFFD/4% hydrogen inhalation; and iv) high hydrogen group, HFFD/67% hydrogen inhalation. After a 10-week experiment, hydrogen inhalation ameliorated weight gain, abdominal fat index, liver index and body mass index of rats fed with HFFD and lowered the total area under the curve in an oral glucose tolerance test. Hydrogen inhalation also ameliorated the increase in liver lipid content and alanine transaminase and aspartate transaminase activities. Liver histopathologic changes evaluated with hematoxylin and eosin as well as Oil Red O staining revealed lower lipid deposition in hydrogen inhalation groups, consistent with the decrease in the expression of the lipid synthesis gene SREBP-1c. The majority of the indicators were affected following treatment with hydrogen in a dose-dependent manner. In conclusion, hydrogen inhalation may play a protective role by influencing the general state, lipid metabolism parameters, liver histology and liver function indicators in the rat model of metabolic syndrome with NAFLD.