GWA Mapping of Anthocyanin Accumulation Reveals Balancing Selection of MYB90 in Arabidopsis thaliana.
GWA Mapping of Anthocyanin Accumulation Reveals Balancing Selection of MYB90 in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0143212
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Keurentjes JJ
中科院分区：
文献类型：
--
作者：
Bac-Molenaar JA;Fradin EF;Rienstra JA;Vreugdenhil D;Keurentjes JJ
以360份拟南芥材料为材料，研究了渗透胁迫对花色素苷积累的诱导作用。广泛的自然变异，从绿色的表型范围内，完全红色/紫色的轮虫，观察。全基因组关联（GWA）作图方法显示，1号染色体上一个小的15 kb区域的序列多样性解释了观察到的变异的40%。对候选基因MYB 90的等位基因的序列和表达分析确定了在花青素苷生物合成途径的该转录因子的氨基酸（AA）位置210处的因果多态性。该氨基酸区分MYB 90的两个最常见的等位基因。这两个等位基因都存在于相当一部分的人口，这表明这两个等位基因之间的平衡选择。对所研究的种质资源的地理来源的分析表明，宏观气候不是花色素苷积累的正面或负面选择背后的驱动力。因此，建议当地气候条件的重要作用。这项研究强调，GWA定位是一个强大的方法来确定等位基因的平衡选择压力的性质。
Induction of anthocyanin accumulation by osmotic stress was assessed in 360 accessions of Arabidopsis thaliana. A wide range of natural variation, with phenotypes ranging from green to completely red/purple rosettes, was observed. A genome wide association (GWA) mapping approach revealed that sequence diversity in a small 15 kb region on chromosome 1 explained 40% of the variation observed. Sequence and expression analyses of alleles of the candidate gene MYB90 identified a causal polymorphism at amino acid (AA) position 210 of this transcription factor of the anthocyanin biosynthesis pathway. This amino acid discriminates the two most frequent alleles of MYB90. Both alleles are present in a substantial part of the population, suggesting balancing selection between these two alleles. Analysis of the geographical origin of the studied accessions suggests that the macro climate is not the driving force behind positive or negative selection for anthocyanin accumulation. An important role for local climatic conditions is, therefore, suggested. This study emphasizes that GWA mapping is a powerful approach to identify alleles that are under balancing selection pressure in nature.