Rhizobacterium Bacillus amyloliquefaciens Strain SQRT3-Mediated Induced Systemic Resistance Controls Bacterial Wilt of Tomato
Rhizobacterium Bacillus amyloliquefaciens Strain SQRT3-Mediated Induced Systemic Resistance Controls Bacterial Wilt of Tomato
复制标题
根际解淀粉芽孢杆菌菌株 SQRT3 介导的诱导系统抗性控制番茄青枯病
DOI：
10.1016/s1002-0160(17)60406-5
复制
发表时间：
2017-12-01
期刊：
影响因子：
5.7
通讯作者：
Shen Qirong
中科院分区：
文献类型：
--
作者：
Li Chunyu;Hu Weicong;Shen Qirong
由青枯菌引起的番茄青枯病严重威胁着世界各地热带和温带番茄的生长。从番茄根际土壤中分离到一株解淀粉芽孢杆菌SQRT 3菌株，该菌株对番茄根际病原菌的生长有较强的抑制作用。青枯菌属在温室条件下，证明了菌株SQRT 3对番茄青枯病的抑制作用。此外，在番茄中诱导的系统抗性（ISR）作为一个潜在的疾病抑制机制进行了研究，在植物接种分离的细菌菌株SQRT 3。结果表明，菌株SQRT 3与R.青枯菌对番茄青枯病的生防效果为68.1%，对番茄青枯病菌的生长抑制率为100%。根际土壤中的青枯菌种群数量与对照相比有显著性差异。青枯菌属菌株SQRT 3经浸根处理后对番茄枯萎病的防效提高到84.1%。用菌株SQRT 3和R.与其它处理相比，青枯菌处理的过氧化物酶和多酚氧化酶活性均有所提高。菌株SQRT 3的应用降低了番茄叶片的膜脂过氧化作用。茉莉酸和水杨酸依赖的信号转导途径的标记基因在SQRT 3和R处理的番茄植株中表达更快更强。与对照相比，R.青枯菌或菌株SQRT 3单独。结果表明，菌株SQRT 3能有效防治番茄枯萎病。
Tomato bacterial wilt caused by Ralstonia solanacearum seriously threats tomato growth in tropical and temperate regions around the world. This study reported an antagonistic bacterial strain, Bacillus amyloliquefaciens strain SQRT3, isolated from the rhizosphere soil of tomato plants, which strongly inhibited in vitro growth of pathogenic R. solanacearum. The suppression of tomato bacterial wilt by strain SQRT3 was demonstrated under greenhouse conditions. Additionally, induced systemic resistance (ISR) in tomato as one of the potential disease suppression mechanisms was investigated in the plants inoculated with the isolated bacterial strain SQRT3. The results showed that strain SQRT3 applied with R. solanacearum by drenching significantly reduced tomato bacterial wilt by 68.1% biocontrol efficiency (BE) and suppressed the R. solanacearum populations in the rhizosphere soil compared to the control only drenched with R. solanacearum. The BE of the isolated bacterial strain SQRT3 against tomato wilt increased to 84.1% by root-dipping. Tomato plants treated with both strain SQRT3 and R. solanacearum showed increases in activities of peroxidase and polyphenol oxidase compared with other treatments. The application of strain SQRT3 reduced membrane lipid peroxidation in tomato leaves. The expressions of marker genes for jasmonic acid- and salicylic acid-dependent signaling pathways were faster and stronger in tomato plants treated with both strain SQRT3 and R. solanacearurn than in plants treated with either R. solanacearum or strain SQRT3 alone. Collectively, the findings indicated that strain SQRT3 can effectively control tomato wilt.