Short-term salinity stress in tobacco plants leads to the onset of animal-like PCD hallmarks in planta in contrast to long-term stress
Short-term salinity stress in tobacco plants leads to the onset of animal-like PCD hallmarks in planta in contrast to long-term stress
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DOI：
10.1007/s00425-009-1060-x
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发表时间：
2010-01-01
期刊：
影响因子：
4.3
通讯作者：
Roubelakis-Angelakis, Kalliopi A.
中科院分区：
文献类型：
--
作者：
Andronis, Efthimios A.;Roubelakis-Angelakis, Kalliopi A.
最近的研究结果表明，线粒体作为中心的压力诱导产生的活性氧在植物中。植物线粒体膜在胁迫过程中的去极化导致细胞质中程序性细胞死亡（PCD）诱导因子的释放，其方式类似于动物类PCD的发生。在此，我们报告显着相似的动物样PCD和盐胁迫诱导的植物PCD。短期盐胁迫（3 h）导致野生型烟草（Nicotiana tabacum cv.）小哈瓦那。然而，PCD没有表现在长期盐胁迫（24小时）。有趣的是，长期盐胁迫导致了坏死样特征，这伴随着呼吸衰竭，呼吸链的关键组分，如CYT-c和交替氧化酶的减少，ATP消耗和高蛋白水解活性。结果表明，烟草植株在植物体内的盐胁迫导致的动物样PCD的发病过程中，只有在早期阶段的压力，而长期的压力导致的坏死样功能。
Recent results have identified mitochondria as centers of stress-induced generation of reactive oxygen species in plants. Depolarization of plant mitochondrial membrane during stress results the release of programmed cell death (PCD)-inducing factors in the cytosol in a fashion similar to the onset of animal-like PCD. Herein, we report significant similarities of animal-like PCD and salinity stress-induced plant PCD. Short-term salinity stress (3 h) led to depolarization of the mitochondrial membrane, release of cytochrome c (CYT-c), which was visualized using a contemporary molecular technique, activation of caspase-3 type proteases and the onset of PCD in wild type tobacco plants, Nicotiana tabacum cv. Petit Havana. However, PCD was not manifested during long-term salinity stress (24 h). Interestingly long-term salinity stress led to necrotic-like features, which were accompanied by collapse of respiration, reduction of key components of the respiratory chain, such as CYT-c and alternative oxidase, ATP depletion and high proteolytic activity. The results suggest that salinity stress of tobacco plants in planta leads to the onset of animal-like PCD only during the early stages post-stress, while long-term stress leads to necrotic-like features.