Controlled release of IGF-1 and HGF from a biodegradable polyurethane scaffold.
Controlled release of IGF-1 and HGF from a biodegradable polyurethane scaffold.
复制标题
DOI：
10.1007/s11095-011-0391-z
复制
发表时间：
2011-06
影响因子：
3.7
通讯作者：
Wagner WR
中科院分区：
文献类型：
--
作者：
Nelson DM;Baraniak PR;Ma Z;Guan J;Mason NS;Wagner WR
可生物降解的弹性体，可以具有良好的机械性能和降解速率的软组织工程应用，最近正在探索作为仓库的生物分子输送。本研究的目的是合成和处理可生物降解的弹性聚（酯氨基甲酸酯）脲（PEUU）支架，并表征其纳入和释放生物活性胰岛素样生长因子-1（IGF-1）和肝细胞生长因子（HGF）的能力。由5或8wt%PEUU制成的多孔PEUU支架用直接生长因子掺入制备。在生理盐水或含有100单位/ml脂肪酶的生理盐水中研究长期体外IGF-1释放动力学以模拟体内降解。使用细胞测定来确认释放的IGF-1和HGF生物活性。IGF-1释放到盐水中以复杂的多相方式发生长达440天。由5重量% PEUU产生的支架比8重量%支架更快地递送蛋白质。对于两种聚合物浓度，脂肪酶加速的支架降解导致在9周内递送>90%的蛋白质。确认了前3周内IGF-1和HGF的生物活性。证明了生物可降解弹性支架提供长期生长因子递送的能力。这样的系统可以在心血管和其他软组织工程应用中提供功能益处。
Biodegradable elastomers, which can possess favorable mechanical properties and degradation rates for soft tissue engineering applications, are more recently being explored as depots for biomolecule delivery. The objective of this study was to synthesize and process biodegradable, elastomeric poly(ester urethane)urea (PEUU) scaffolds and to characterize their ability to incorporate and release bioactive insulin-like growth factor–1 (IGF-1) and hepatocyte growth factor (HGF). Porous PEUU scaffolds made from either 5 or 8 wt% PEUU were prepared with direct growth factor incorporation. Long-term in vitro IGF-1 release kinetics were investigated in saline or saline with 100 units/ml lipase to simulate in vivo degradation. Cellular assays were used to confirm released IGF-1 and HGF bioactivity. IGF-1 release into saline occurred in a complex multi-phasic manner for up to 440 days. Scaffolds generated from 5 wt% PEUU delivered protein faster than 8 wt% scaffolds. Lipase-accelerated scaffold degradation lead to delivery of >90% protein over 9 weeks for both polymer concentrations. IGF-1 and HGF bioactivity in the first 3 weeks was confirmed. The capacity of a biodegradable elastomeric scaffold to provide long-term growth factor delivery was demonstrated. Such a system might provide functional benefit in cardiovascular and other soft tissue engineering applications.