Effect of introduced Pseudomonas fluorescens strains on the uptake of nitrogen by wheat from 15N-enriched organic residues
Effect of introduced Pseudomonas fluorescens strains on the uptake of nitrogen by wheat from 15N-enriched organic residues
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DOI：
10.1023/a:1008999112121
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发表时间：
1999-08-01
影响因子：
4.1
通讯作者：
Lynch, JM
中科院分区：
文献类型：
--
作者：
Brimecombe, MJ;De Leij, FAAM;Lynch, JM
在微宇宙研究中，研究了携带标记基因盒lacZY的产芽孢杆菌荧光假单胞菌菌株（F113）和标记的非产芽孢杆菌菌株（F113 G22）对掺入桑迪土壤中的富含N-15的有机残留物的氮吸收的影响。菌株F113产生抗生素2，4-二乙酰间苯三酚（DAPG），而其修饰的衍生菌株F113 G22通过Tn 5诱变缺失了DAPG产生。利用稳定同位素比质谱法测定了17天龄植株的地上部和根系物质，研究了小麦（Triticum aestivum）从富含N-15的有机残留物中吸收氮的情况。此外，还监测了植物生长和土壤中活性微生物生物量。与我们以前对豌豆（豌豆）的研究结果相反，发现在小麦中，接种菌株F113或F113 G22与未接种对照相比，降低了来自N-15标记的有机残留物掺入土壤中的氮的比例。因此，这表明，这些菌株降低矿化的有机残留物在小麦根际，使较少的无机N（N-15）可供植物吸收。本研究的结果表明，引入荧光假单胞菌菌株对根际氮矿化的影响是植物物种依赖的，并强调了在一系列植物物种上测试微生物接种物的重要性。
The effects of an antibiotic-producing Pseudomonas fluorescens strain (F113) carrying the marker gene cassette lacZY and a marked, non-producing strain (F113G22) on the uptake of nitrogen from N-15-enriched organic residues incorporated into a sandy soil were investigated in microcosm studies. Strain F113 produces the antibiotic 2,4-diacetylphloroglucinol (DAPG), whilst its modified derivative strain F113G22 has DAPG production deleted by Tn5 mutagenesis. Uptake of nitrogen by wheat (Triticum aestivum) from N-15-enriched organic residues was estimated using stable isotope-ratio mass spectrometry of shoot and root material of 17-day-old plants. In addition, plant growth and active microbial biomass in soil were monitored. In contrast to results obtained in our previous study on pea (Pisum sativum), it was found that in wheat, inoculation with either strain F113 or F113G22 decreased the proportion of nitrogen derived from N-15-labelled organic residues incorporated into soil as compared to non-inoculated controls. It is therefore suggested that these strains decreased mineralization of organic residues in the rhizosphere of wheat, making less inorganic N (N-15) available for plant uptake. The results of this study indicate that the effects of introduced Pseudomonas fluorescens strains on nitrogen mineralization in the rhizosphere are plant-species dependent, and highlight the importance of testing microbial inocula on a range of plant species.