Primary adhesion of Enteromorpha (Chlorophyta, Ulvales) propagules: Quantitative settlement studies and video microscopy
Primary adhesion of Enteromorpha (Chlorophyta, Ulvales) propagules: Quantitative settlement studies and video microscopy
复制标题
DOI：
10.1111/j.0022-3646.1997.00938.x
复制
发表时间：
1997-12-01
影响因子：
2.9
通讯作者：
Wetherbee, R
中科院分区：
文献类型：
--
作者：
Callow, ME;Callow, JA;Wetherbee, R
定量方法和相关的动力学分析已被用于第一次研究各种类型的浒苔繁殖体的沉降和粘附的详细方面。时程实验表明，quadri和biflagellate游动孢子和受精卵粘附迅速，但在任何一个人口的比例似乎是不称职的粘附到底层。在游动孢子浓度范围内进行的粘附实验的动力学（Scatchard）分析显示，密度依赖性的影响，以前没有报道过，在低孢子密度和高孢子密度的负协同性在低协同性。高分辨率视频显微镜首次用于揭示游动孢子和合子的定居和粘附的各个阶段的细节。新的观察，最初的，临时阶段的附件通过顶端乳头，随后由一个永久的承诺阶段，其特征在于放电的含粘合剂的细胞质囊泡，作为对表面的细胞收缩，并吸附鞭毛。承诺阶段之后，通过在界面处的变形虫样运动来利用表面。群居定居行为经常被观察到，导致筏状细胞的形成。讨论了密度依赖型孢子粘附的可能机制和意义。
Quantitative methods and associated kinetic analyses have been used for the first time to study detailed aspects of the settlement and adhesion of various types of Enteromorpha propagule. Time course experiments showed that quadri- and biflagellate zoospores and zygotes adhered rapidly, but a proportion within any one population appeared to be incompetent at adhering to the substratum. Kinetic (Scatchard) analysis of adhesion experiments performed at a range of zoospore concentrations revealed density-dependent effects not previously reported, with positive cooperativity at low spore densities and negative cooperativity at high spore densities. High-resolution video microscopy was used for the first time to reveal details of the various stages in the settlement and adhesion of zoospores and zygotes. Novel observations were made of an, initial, temporary phase of attachment via the apical papilla, followed by a permanent phase of commitment, characterized by discharge of adhesive-containing cytoplasmic vesicles, as the cell contracted against the surface, and adsorption of flagella. The Phase of commitment was followed by exploitation of the surface through amoeboid-like movements at the interface. Gregarious settlement behavior was frequently observed leading to the formation of rafts of cells. The possible mechanisms and significance of density-dependent spore adhesion are discussed.