DNA helicase-mediated packaging of adeno-associated virus type 2 genomes into preformed capsids
DNA helicase-mediated packaging of adeno-associated virus type 2 genomes into preformed capsids
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DOI：
10.1093/emboj/20.12.3282
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发表时间：
2001-06-15
期刊：
影响因子：
11.4
通讯作者：
Kleinschmidt, JA
中科院分区：
文献类型：
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作者：
King, JA;Dubielzig, R;Kleinschmidt, JA
解旋酶不仅催化核酸互补区域之间氢键的破坏，而且以极性方式沿着核酸链移动。在这里，我们表明，Rep 52和Rep 40蛋白的腺相关病毒2型（AAV-2）所需的易位衣壳相关，单链DNA基因组到预先形成的空AAV-2衣壳，和Rep 52/40的DNA解旋酶功能是必不可少的这个过程。此外，DNA酶保护实验表明，AV-2基因组的插入从3'端开始，这与Rep 52/40解旋酶所证明的3'->5'持续合成能力相关。提出了一个模型，其中衣壳固定化解旋酶复合物充当分子马达将单链DNA“泵送”穿过衣壳边界。
Helicases not only catalyse the disruption of hydrogen bonding between complementary regions of nucleic acids, but also move along nucleic acid strands in a polar fashion. Here we show that the Rep52 and Rep40 proteins of adeno-associated virus type 2 (AAV-2) are required to translocate capsid-associated, single-stranded DNA genomes into preformed empty AAV-2 capsids, and that the DNA helicase function of Rep52/40 is essential for this process. Furthermore, DNase protection experiments suggest that insertion of AAV-2 genomes proceeds from the 3' end, which correlates with the 3'-->5' processivity demonstrated for the Rep52/40 helicase, A model is proposed in which capsid-immobilized helicase complexes act as molecular motors to 'pump' single-stranded DNA across the capsid boundary.