Increased concentration of thymidine kinase in rat hepatomas.
Increased concentration of thymidine kinase in rat hepatomas.
复制标题
大鼠肝癌中胸苷激酶浓度增加。
DOI：
10.1016/s0006-291x(83)80148-x
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Weber,G
中科院分区：
文献类型：
--
作者：
Lai,MH;Weber,G
用亲和层析法从大鼠肝癌3924A细胞溶质组分中纯化出接近均一的胸苷激酶。该酶的MR为81,000，由MR=44,000的两个亚基组成。抗其抗血清可中和大鼠肝脏和肝癌组织中胸苷激酶的活性。抗血清中和实验表明，慢、中、快生长的肝癌16、7787和3924A的肝细胞浆胸苷激酶含量分别增加了4倍、15倍和25倍。
Thymidine kinase was purified to near homogeneity by affinity chromatography from cytosol fraction of rat hepatoma 3924A. The enzyme had a Mr of 81,000 and was composed of two subunits of Mr = 44,000. Antiserum made against it neutralized the activities of thymidine kinase from both rat livers and hepatomas. Neutralization studies with the antiserum revealed that hepatic transformation resulted in 4-, 15- and 25-fold increase in the amount of cytosol thymidine kinase in hepatomas 16, 7787 and 3924A of slow, medium and fast growth rate, respectively.