Elevation of plasma neurotensinlike immunoreactivity after a meal. Characterization of the elevated components.
Elevation of plasma neurotensinlike immunoreactivity after a meal. Characterization of the elevated components.
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餐后血浆神经降压素样免疫反应性升高。
DOI：
10.1172/jci110605
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Leeman,SE
中科院分区：
文献类型：
--
作者：
Hammer,RA;Carraway,RE;Leeman,SE
餐后数小时内外周血浆中神经降压素样免疫反应性的升高已被证实，这主要是由于神经降压素 (NT) 氨基末端片段的存在，而不是 NT 本身。我们开发了一种程序来分离和表征这些 N 端交叉反应物质，并估计这些成分对血浆神经降压素样免疫反应性的贡献。对合并的血浆提取物在 Sephadex G-25 上进行凝胶色谱分析，然后进行反相高压液相色谱分析，结果表明血浆中存在与 NT 及其 N 末端部分序列 NT(1-8) 和 NT(1-11) 共洗脱的肽。在五项实验中，对特定膳食之前和之后 1 小时收集的血浆进行比较，结果表明 C 端免疫反应性没有变化，而 N 端免疫反应性增加了 8 至 10 倍。四项实验中餐前和餐后混合血浆的色谱分析表明，使用 N 端定向抗血清测得的神经降压素样免疫反应性的所有升高基本上都是由于 NT(1-8) 和 NT(1-11) 的增加所致，而使用 C 端定向抗血清测量的 NT 本身在给药后 1 小时在外周血浆中并未明显增加。 一顿饭。 [3H]NT与全血在体外孵育后确实产生了与NT(1-8)和NT(1-11)具有相同洗脱时间的氚化物质，这为这些片段是NT的代谢物提供了支持证据。这些片段循环水平的显着升高反映了测试餐后 NT 分泌的增加。分泌的 NT 在被代谢之前可能已充当激素，或者可能仅具有局部（旁分泌）作用。
The detection of an elevation in neurotensinlike immunoreactivity in peripheral plasma for several hours after a meal has been confirmed and shown to be primarily due to the presence of aminoterminal fragments of neurotensin (NT) rather than to NT itself. We have developed a procedure to separate and characterize these N-terminal cross-reacting substances, and to estimate the contributions of these constitutents to plasma neurotensinlike immunoreactivity. Gel chromatography of pooled plasma extracts on Sephadex G-25 followed by reverse-phase high pressure liquid chromatography indicated that peptides coeluting with NT and its N-terminal partial sequences NT(1-8) and NT(1-11) were present in plasma. Comparison of plasmas collected before and 1 h after a defined meal, in five experiments, demonstrated no change in C-terminal immunoreactivity and an 8- to 10-fold rise in N-terminal immunoreactivity. Chromatographic analysis of pooled pre- and postmeal plasma in four experiments showed that essentially all of this elevation in neurotensinlike immunoreactivity measured with an N-terminal directed antiserum was due to increases in NT(1-8) and NT(1-11), while NT itself, measured using a C-terminal directed antiserum, did not increase appreciably in peripheral plasma 1 h after the meal. Generation of tritiated substances with the same elution times as NT(1-8) and NT(1-11) did occur after incubation of [3H]NT with whole blood in vitro, providing supporting evidence that these fragments are metabolites of NT. The marked elevation in the circulating levels of these fragments reflects that an increased secretion of NT occurred in response to the test meal. The secreted NT may have acted as a hormone before it was metabolized, or it may only have had a local (paracrine) effect.
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神经降压素（一种下丘脑肽）的放射免疫测定。
DOI：
10.1016/s0021-9258(17)32937-x
发表时间：
1976
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Carraway;S. Leeman
通讯作者：
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DOI：
10.1016/s0021-9258(19)41780-8
发表时间：
1975
期刊：
The Journal of biological chemistry
影响因子：
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作者：
R. Carraway;S. Leeman
通讯作者：
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狗肠粘膜中存在免疫反应性神经降压素的证据。
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作者：
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影响因子：
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作者：
R. Carraway;R. Hammer;S. Leeman
通讯作者：
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