CHARACTERIZATION OF THE CHROMOSOMAL PROTEIN MC1 FROM THE THERMOPHILIC ARCHAEBACTERIUM METHANOSARCINA SP CHTI 55 AND ITS EFFECT ON THE THERMAL-STABILITY OF DNA
CHARACTERIZATION OF THE CHROMOSOMAL PROTEIN MC1 FROM THE THERMOPHILIC ARCHAEBACTERIUM METHANOSARCINA SP CHTI 55 AND ITS EFFECT ON THE THERMAL-STABILITY OF DNA
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DOI：
10.1016/0167-4781(88)90035-8
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发表时间：
1988-11-10
期刊：
影响因子：
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通讯作者：
SAUTIERE, P
中科院分区：
文献类型：
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作者：
CHARTIER, F;LAINE, B;SAUTIERE, P
在甲烷八叠球菌CHTI 55的脱氧核糖核蛋白复合物中，DNA与两种蛋白质结合，命名为MC 1（产甲烷菌染色体蛋白1）（Mr 10760）和MC 2（Mr 17000）。这些蛋白质中最丰富的蛋白质MC1与巴氏甲烷八叠球菌MS蛋白质MC1密切相关。研究了甲烷八叠球菌CHTI 55蛋白质MC 1对天然脱氧核糖核蛋白复合物以及重组复合物中DNA热稳定性的影响，并与E. coli DNA结合蛋白II。这两种蛋白质都能够保护DNA免受热变性，但在熔解曲线中观察到的差异表明它们通过不同的机制相互作用。此外，我们的研究表明，一个MC1蛋白分子可以保护8个碱基对的DNA。
In the deoxyribonucleoprotein complex of Methanosarcina sp. CHTI 55, DNA is associated with two proteins, named MC1 (methanogen chromosomal protein 1), (Mr 10760) and MC2 (Mr 17000). Protein MC1, the most abundant of these proteins, is closely related to the Methanosarcina barkeri MS protein MC1. The effect of Methanosarcina sp. CHTI 55 protein MC1 on the thermal stability of DNA has been studied in native deoxyribonucleoprotein complex, as well as in reconstituted complexes, and it has been compared to the effect of E. coli DNA-binding protein II. Both proteins are able to protect DNA against thermal denaturation, but the differences observed in the melting profiles suggest that they interact by different mechanisms. Moreover, our studies indicate that one molecule of protein MC1 protects eight base pairs of DNA.