Different miR-21-3p isoforms and their different features in colorectal cancer
Different miR-21-3p isoforms and their different features in colorectal cancer
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不同的miR-21-3p亚型及其在结直肠癌中的不同特征
DOI：
10.1002/ijc.30902
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发表时间：
2017-11-15
影响因子：
6.4
通讯作者：
Li, Jianming
中科院分区：
文献类型：
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作者：
Jiao, Weijuan;Leng, Xueqin;Li, Jianming
miR-21是目前发现的唯一一种在实体瘤中过表达的microRNA，其引导链miR-21- 5 p在结直肠癌中的研究较多，而其过客链miR-21- 3 p的研究较少。本研究基于美国癌症基因组图谱（TCGA）数据，发现微卫星不稳定（MSI）型大肠癌中miR-21- 3 p异构体的种类和数量较多。我们通过体外和体内研究进一步检查了miR-21- 3 p的作用。miR-21- 3 p可能是CRC中的癌基因，通过上皮-间充质转化促进细胞移动。然而，不同的亚型，特别是miR-21- 3 p 0竖条2可能是CRC生存的有利预后标志物，这可能是由于miR-21- 5 p和/或靶基因的互补效应增加。需要进一步研究miRNA亚型的潜在机制。
MiR-21, the only microRNA (miRNA) found to be overexpressed in any type of solid tumor, its guide stand, miR-21-5p, has been studied a lot in colorectal cancer (CRC); however, few researchers focused on its passenger strand, miR-21-3p. In our study, based on The Cancer Genome Atlas (TCGA) data, we found that there were more varieties and quantities of miR-21-3p isoforms in microsatellite instability (MSI)-type CRC. We further examined the role of miR-21-3p by in vitro and in vivo studies. MiR-21-3p may be an oncogene in CRC by promoting cellular mobility through epithelial-mesenchymal transition. However, different isoforms, especially miR-21-3p 0 vertical bar 2 may be a favorable prognostic marker for CRC survival, probably due to increased complementary effect of miR-21-5p and/or target genes. Further study investigating the underlying mechanism of miRNA isoforms is needed.