TENASCIN IN THE INJURED RAT OPTIC-NERVE AND IN NONNEURONAL CELLS IN-VITRO - POTENTIAL ROLE IN NEURAL REPAIR
TENASCIN IN THE INJURED RAT OPTIC-NERVE AND IN NONNEURONAL CELLS IN-VITRO - POTENTIAL ROLE IN NEURAL REPAIR
复制标题
DOI：
10.1002/cne.903400208
复制
发表时间：
1994-02-08
影响因子：
2.5
通讯作者：
DAVID, S
中科院分区：
文献类型：
--
作者：
AJEMIAN, A;NESS, R;DAVID, S
应用免疫荧光双标记技术，观察了腱生蛋白在体外损伤的成年大鼠视神经和中枢神经系统（CNS）非神经元细胞中的分布。腱生蛋白样免疫反应定位于软脑膜和星形胶质细胞边界的网站的视神经横断。早在横切后24小时就观察到抗腱生蛋白标记，当时它出现在软脑膜和神经组织之间的精细界面。在接下来的2周内，该边界区域的细胞中抗腱生蛋白标记增加，并在横切后18-21天消失。体外研究进一步证实，星形胶质细胞和软脑膜细胞都表达腱生蛋白，如通过用抗腱生蛋白抗体进行免疫荧光标记所检测的。然而，与两种细胞类型相关的免疫标记模式不同。星形胶质细胞的细胞表面显示出专门的点状标记，而软脑膜细胞主要表现出粗糙，dumerary，基质样存款。星形胶质细胞和软脑膜细胞保持分离时，共培养。在这些培养物中，当这两种细胞类型彼此接触时，在星形胶质细胞和软脑膜细胞之间的界面区域中也观察到腱生蛋白的基质样沉积物的量增加。考虑到腱生蛋白的抗粘附和抗扩散特性，这些体内和体外结果表明，腱生蛋白可能在损伤后的前2周期间在CNS创伤部位的软脑膜细胞与神经组织的初始分离中起作用，即，在完全分化的胶质界膜形成之前。因此，腱生蛋白可能影响胶质界膜形成的早期阶段，从而防止损伤后软脑膜细胞不加选择地迁移到CNS组织中。(C)1994 Wiley-Liss，Inc.
The distribution of tenascin was examined in the lesioned adult rat optic nerve and central nervous system (CNS) non-neuronal cells in vitro, by means of a double immunofluorescence technique. Tenascin-like immunoreactivity is localized to the leptomeninges and astrocytes that border the site of optic nerve transection. Anti-tenascin labeling was observed as early as 24 hours after transection, when it appeared as a fine interface between leptomeninges and neural tissue. The anti-tenascin labeling increased in the cells at this border zone during the next 2 weeks, and disappeared 18-21 days after transection. In vitro studies further confirmed that both astrocytes and leptomeningeal cells express tenascin as detected by immunofluorescence labeling with anti-tenascin antibodies. However, the pattern of immunolabeling associated with the two cell types differed. Astrocytes showed exclusively punctate labeling of the cell surface, while leptomeningeal cells showed mainly coarse, fibrillary, matrix-like deposits. Astrocytes and leptomeningeal cells remained,segregated when cocultured. In these cultures, an increased amount of the fibrillary, matrix-like deposits of tenascin was also observed in the region of the interface between astrocytes and leptomeningeal cells when these two cell types contact each other. Given the antiadhesive and antispreading properties of tenascin, these in vivo and in vitro results suggest that tenascin might play a role in the initial segregation of leptomeningeal cells from neural tissue at the site of CNS trauma during the first 2 weeks after injury, i.e., prior to the formation of a fully differentiated glia limitans. Therefore, tenascin may influence the early stages in the formation of the glia limitans, and thus prevent the indiscriminate migration of leptomeningeal cells into CNS tissue after injury. (C) 1994 Wiley-Liss, Inc.