Induction of astrocytic nestin expression by depolarization in rats
Induction of astrocytic nestin expression by depolarization in rats
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DOI：
10.1016/s0304-3940(01)02292-3
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发表时间：
2001-11-16
影响因子：
2.5
通讯作者：
Mathiesen, T
中科院分区：
文献类型：
--
作者：
Holmin, S;von Gertten, C;Mathiesen, T
巢蛋白在中枢神经系统 (CNS) 祖细胞中表达，其在成熟细胞中的表达代表在细胞应激下向分化程度较低的细胞状态的转变。本研究旨在证实巢蛋白合成是由去极化诱导且依赖于 N-甲基-D-天冬氨酸 (NMDA) 受体激活的假设。通过在暴露的大鼠皮层上施用氯化钾来诱导去极化，并通过免疫组织化学评估巢蛋白表达。去极化诱导星形细胞巢蛋白表达，该表达是局部的，或者在整个同侧皮质中明显，具体取决于暴露时间。巢蛋白表达依赖于 NMDA 受体，因为 MK-801 治疗消除了这种反应。了解巢蛋白表达的机制很重要，因为这种蛋白在反应性和分化程度较低的中枢神经系统细胞状态以及神经干细胞中表达。对巢蛋白表达控制的深入了解还可以提供在创伤/缺血后或移植策略中控制中枢神经系统细胞分化的方法。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Nestin is expressed in central nervous system (CNS) progenitor cells and its expression in mature cells represents transition to a less differentiated cellular state under cellular stress. This study was performed to corroborate the hypothesis that nestin synthesis is induced by depolarization and dependent on N-methyl-D-aspartate (NMDA)-receptor activation. Depolarization was induced with application of potassium chloride on the exposed rat cortex and nestin expression was evaluated by immunohistochemistry. Depolarization induced astrocytic nestin expression that was local, or evident in the entire ipsilateral cortex depending on the time of exposure. Nestin expression was NMDA-receptor-dependent since MK-801 treatment abolished the response. Understanding the mechanisms for nestin expression is important since this protein is expressed in reactive and less differentiated CNS cell states and also in neural stem cells. Insights into the control of nestin expression may also provide means for controlling differentiation of CNS cells either post-trauma/ischemia or in transplantation strategies. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.