Differences in hypervariable region 1 quasispecies of hepatitis C virus between human serum and peripheral blood mononuclear cells.
Differences in hypervariable region 1 quasispecies of hepatitis C virus between human serum and peripheral blood mononuclear cells.
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人血清与外周血单个核细胞丙型肝炎病毒高变区1准种的差异。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
K. Okita
中科院分区：
文献类型：
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作者：
K. Fujii;K. Hino;M. Okazaki;M. Okuda;S. Kondoh;K. Okita
对11例慢性丙型肝炎患者血清和外周血单个核细胞（PBMCs）中丙型肝炎病毒（HCV）高变区1（HVR 1）准种的复杂性进行了研究。聚合酶链反应和单链构象多态性分析显示，4例（36%）患者血清和PBMC中HVR 1准种的复杂性发生了系列性变化，2例（18%）患者血清和PBMC中的准种既发生了系列性变化，又存在准种差异; 5例（45%）患者血清和PBMC之间既无系列变化，也无准种差异。这些结果表明，血清和PBMC之间存在不同的准种和PBMC的HCV感染。
The complexity of hypervariable region 1 (HVR1) quasispecies of hepatitis C virus (HCV) in serum and peripheral blood mononuclear cells (PBMCs) was assessed at several time points in 11 patients with chronic hepatitis C. Polymerase chain reaction and single-strand conformation polymorphism analysis revealed that four patients (36%) showed serial changes in the complexity of HVR1 quasispecies, which were identical in both serum and PBMCs; two patients (18%) showed both serial changes in quasispecies and differences in quasispecies between serum and PBMCs; and five patients (45%) showed neither serial changes in nor quasispecies differences between serum and PBMCs. These results demonstrate both the existence of different quasispecies between serum and PBMCs and the infection of PBMCs by HCV.