Identification of potential markers for internal exposure to ambient ozone in oral cavity of healthy adults.
Identification of potential markers for internal exposure to ambient ozone in oral cavity of healthy adults.
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DOI：
10.1016/j.envres.2020.109907
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发表时间：
2020-08
影响因子：
8.3
通讯作者：
D. Hu;L. Cui;Y. Qi;X. Jia;Jiahui Chen;W. Niu;Mark R Miller;M. Loh;Hui Zhou;F. Deng-
中科院分区：
文献类型：
--
作者：
D. Hu;L. Cui;Y. Qi;X. Jia;Jiahui Chen;W. Niu;Mark R Miller;M. Loh;Hui Zhou;F. Deng-
臭氧是一种与不良健康结果相关的高度氧化性气体污染物，但内部暴露于环境臭氧的标志物尚未建立。方法评价口腔微生物组标志物对环境臭氧暴露的适用性和可行性。2018年3月至5月期间，对97名健康成年人进行了2至3次口腔拭子取样检查。采集采样前1-7天的每小时环境臭氧浓度。采用混合效应模型研究了环境臭氧与口腔微生物群多样性和分类丰度之间的关系。受试者工作特征（ROC）曲线估计了标记物在暴露于不同浓度环境臭氧的样品之间的准确性。maker和肺功能之间的关系通过线性混合效应模型进一步检验。结果大气臭氧日平均值（O3-daily）、最大8h平均值（O3-8hmax）和1h最大值（O3-1hmax）分别为72、123和144 μg/m³。o3 -daily与口腔微生物α-多样性呈正相关，但暴露-响应曲线仅在o3 -daily 60 ~ 75 μg/m³范围内呈正相关。o3 - 8hmax3和o3 - 1hmax3与O3-daily的结果一致。随着o3 - daily04的四分位数范围的增加，变形菌门的丰度下降了3.1% (95% CI: - 4.0%, - 2.2%)，厚壁菌门的丰度增加了3.3% (95% CI: 2.3%, 4.3%)，而变形菌门：厚壁菌门的比值（P/F）下降了0.9 （95% CI: - 1.5, - 0.4）。变形菌门（proteobacteria）、厚壁菌门（firmicutes）和P/F曲线下面积分别为0.8535、0.7569和0.8929。变形菌群和P/F与第一秒用力呼气量和部分呼出一氧化氮显著相关。结论环境臭氧扰乱口腔微生物稳态。变形菌、厚壁菌及其比例可能是短期环境臭氧暴露的潜在标志，也是气道炎症或肺功能下降的指标。
BackgroundOzone is a highly oxidative gaseous pollutant associated with adverse health outcomes, but markers for internal exposure to ambient ozone are not well-established.MethodsWe aimed to evaluate the feasibility and suitability of the markers in oral microbiome for ambient ozone exposure. Between March and May in 2018, 97 healthy adults were examined on 2 or 3 occasions for oral swab sampling. Hourly concentrations of ambient ozone 1–7 days preceding sampling were collected. Mixed-effect models were fitted to examine the associations between ambient ozone and the diversity and taxon abundances of oral microbiome. Receiver operating characteristic (ROC) curves estimated the accuracies of markers to delineate between samples exposed to different concentrations of ambient ozone. The associations between the makers and lung function were further examined by linear mixed effect models.ResultsThe averages of daily mean concentrations of ambient ozone (O3-daily), maximum 8-h means (O3-8hmax) and 1-h maximums (O3-1hmax) were respectively 72 μg/m³, 123 μg/m³ and 144 μg/m³. O3-dailywas positively associated with α-diversity of oral microbiome, but the exposure-response curves only yielded positive associations in the range of O3-dailyfrom 60 μg/m³ to 75 μg/m³. Results of O3-8hmaxand O3-1hmaxwere consistent with these of O3-daily. With an interquartile range increase in O3-dailyat lag04, the abundance of Proteobacteria decreased by 3.1% (95% CI: −4.0%, −2.2%) and Firmicutes increased by 3.3% (95% CI: 2.3%, 4.3%), whilst theProteobacteria:Firmicutes ratio (P/F) decreased by 0.9 (95% CI: −1.5, −0.4). The areas under ROC curves forProteobacteria,Firmicutesand P/F were 0.8535, 0.7569 and 0.8929, respectively.Proteobacteriaand P/F were associated with forced expiratory volume in the first second and fractional exhaled nitric oxide significantly.ConclusionAmbient ozone disturbs oral microbial homeostasis.Proteobacteria,Firmicutesand their ratio may be potential markers for short-term ambient ozone exposure, and indicators of airway inflammation or lung function decline.