Transforming growth factor-beta 1, 2, 3 and receptor type I and II in diabetic foot ulcers
Transforming growth factor-beta 1, 2, 3 and receptor type I and II in diabetic foot ulcers
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DOI：
10.1046/j.1464-5491.2002.00692.x
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发表时间：
2002-06-01
影响因子：
3.5
通讯作者：
Ferguson, MWJ
中科院分区：
文献类型：
--
作者：
Jude, EB;Blakytny, R;Ferguson, MWJ
目的应用免疫组织化学、免疫荧光和Western印迹技术研究转化生长因子β 1、2、3和TGF β受体I、II在糖尿病足溃疡、糖尿病皮肤和正常皮肤中的分布。我们还比较了TGF-β与慢性静脉性溃疡。方法皮肤活检，从非糖尿病和糖尿病受试者的腿或脚，并从糖尿病足溃疡和慢性静脉性溃疡的边缘。TGF-β 1、2和3以及TGF-β受体（RI和RII）的分布（通过免疫荧光和免疫细胞化学）通过使用适当的抗体对8 μ m皮肤切片染色来进行。结果TGF-β在糖尿病足溃疡边缘上皮中的表达明显高于糖尿病足和正常皮肤（P = 0.03，0.02），静脉性溃疡中TGF-β的表达也高于正常皮肤。然而，TGF-β 1的表达在糖尿病足溃疡和慢性静脉溃疡中没有增加，并且与糖尿病和正常皮肤相当。结论糖尿病足溃疡和静脉性溃疡中TGF-β 1的上调可能解释了这些慢性伤口愈合不良的原因，并可能代表了慢性化的一般模式。
Aims To study the distribution of transforming growth factor-beta (TGF-beta) 1, 2 and 3, and TGF-beta receptor types I and II in diabetic foot ulcers, diabetic skin and normal skin by immunohistochemistry, immunofluorescence and Western blotting. We also compared the TGF-betas with those of chronic venous ulcers.Methods Skin biopsies were obtained from the leg or the foot of non-diabetic and diabetic subjects, and from the edge of diabetic foot ulcers and chronic venous ulcers. Distribution (by immunofluorescence and immunocytochemistry) of TGF-beta 1, 2 and 3 and TGF-beta receptors (RI and RII) was done by staining 8-mum skin sections using appropriate antibodies. Protein levels of TGF-beta were measured by Western blot analysis.Results TGF-beta expression was increased in the epithelium at the edge of diabetic foot ulcers, being more intense than diabetic and normal skin (P = 0.03, 0.02, respectively), as was its expression in venous ulcers compared with normal skin. However, TGF-beta1 expression was not increased in diabetic foot ulcers and chronic venous ulcers, and was comparable to diabetic and normal skin. There was also no increase for the receptors in diabetic foot ulcers.Conclusion The lack of TGF-beta1 up-regulation in both diabetic foot ulcers and venous ulcers may explain the impaired healing in these chronic wounds, and could represent a general pattern for chronicity.