Association of rs5888 SNP in the scavenger receptor class B type 1 gene and serum lipid levels.
Association of rs5888 SNP in the scavenger receptor class B type 1 gene and serum lipid levels.
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DOI：
10.1186/1476-511x-11-50
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发表时间：
2012-07-09
影响因子：
4.5
通讯作者：
Pan SL
中科院分区：
文献类型：
--
作者：
Wu DF;Yin RX;Hu XJ;Aung LH;Cao XL;Miao L;Li Q;Yan TT;Wu JZ;Pan SL
白裤瑶是我国瑶族的一个特殊族群。本研究旨在探讨广西白裤瑶族和汉族人群清道夫受体B 1型（scavenger receptor class B type 1，SCARB 1）基因rs 5888单核苷酸多态性（single nucleotide polymorphism，SNP）及环境因素与血脂水平的关系。从分层随机整群抽样中随机抽取白裤瑶族598人和汉族585人。采用聚合酶链反应-限制性片段长度多态性结合凝胶电泳技术对SCARB 1 rs 5888 SNP进行基因分型，并通过直接测序进行验证。白裤瑶组总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、载脂蛋白（Apo）AI均低于汉族组，而ApoB高于汉族组（P < 0.05-0.001）。白裤瑶组C和T等位基因频率分别为78.3%和21.7%，汉族组分别为73.7%和26.3%（P < 0.01）。白裤瑶组CC、CT和TT基因型频率分别为60.0%、36.6%和3.4%，汉族组分别为54.2%、39.0%和6.8%（P < 0.01）。两个民族中TT基因型者HDL-C和ApoAI水平均低于CC和CT基因型者（均P < 0.05）。亚组分析显示，白裤瑶TT基因型男性HDL-C和ApoAI水平低于CC和CT基因型（均P < 0.05），女性T等位基因携带者TC、LDL-C和ApoB水平高于T等位基因非携带者（均P < 0.05）。汉族TT基因型男性HDL-C和ApoAI水平也低于CC和CT基因型，但差异无统计学意义（分别为P = 0.063和P = 0.086）。多元线性回归分析证实了两个种族中血清HDL-C和ApoAI水平与基因型的相关性。血脂参数也与一些环境因素相关。两个民族之间血脂水平的差异可能部分归因于SCARB 1 rs 5888 SNP和一些环境因素的差异。
Bai Ku Yao is a special subgroup of the Yao minority in China. The present study was undertaken to detect the association of rs5888 single nucleotide polymorphism (SNP) in the scavenger receptor class B type 1 (SCARB1) gene and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. A total of 598 subjects of Bai Ku Yao and 585 subjects of Han Chinese were randomly selected from our stratified randomized cluster samples. Genotypes of the SCARB1 rs5888 SNP were determined by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. The levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) AI were lower but ApoB was higher in Bai Ku Yao than in Han (P < 0.05-0.001). The frequencies of C and T alleles were 78.3% and 21.7% in Bai Ku Yao, and 73.7% and 26.3% in Han (P < 0.01); respectively. The frequencies of CC, CT and TT genotypes were 60.0%, 36.6% and 3.4% in Bai Ku Yao, and 54.2%, 39.0% and 6.8% in Han (P < 0.01); respectively. The subjects with TT genotype in both ethnic groups had lower HDL-C and ApoAI levels than the subjects with CC or CT genotype (P < 0.05 for all). Subgroup analyses showed that the subjects with TT genotype in Bai Ku Yao had lower HDL-C and ApoAI levels in males than the subjects with CC or CT genotype (P < 0.05 for all), and the T allele carriers had higher TC, LDL-C and ApoB levels in females than the T allele noncarriers (P < 0.05 for all). The participants with TT genotype in Han also had a lower tendency of HDL-C and ApoAI levels in males than the participants with CC or CT genotype, but the difference did not reach statistically significant (P = 0.063 and P = 0.086; respectively). The association of serum HDL-C and ApoAI levels and genotypes was confirmed by the multiple linear regression analysis in both ethnic groups. Serum lipid parameters were also correlated with several environmental factors. The differences in serum lipid levels between the two ethnic groups might partially attribute to the differences in the SCARB1 rs5888 SNP and several environmental factors.