Cold-Induced Exodus of Postsynaptic Proteins From Dendritic Spines
Cold-Induced Exodus of Postsynaptic Proteins From Dendritic Spines
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DOI：
10.1002/jnr.21852
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发表时间：
2009-02-01
影响因子：
4.2
通讯作者：
Chang, Yen-Chung
中科院分区：
文献类型：
--
作者：
Cheng, Hui-Hsuan;Huang, Zu-Han;Chang, Yen-Chung
树突棘是神经元树突上的小突起，是哺乳动物大脑兴奋性输入的主要靶点。培养的神经元和脑片是研究树突棘的生化和细胞特性的重要工具。在对神经元进行免疫细胞化学研究和制备脑片的过程中，神经元经常被保存在低于37摄氏度的温度下不同的时间。这项研究试图调查冷处理是否以及如何影响树突棘的蛋白质组成。结果表明，冷处理后，培养的大鼠海马神经元树突棘中的4种突触后蛋白，即α、β-微管蛋白、钙、钙调蛋白依赖的蛋白激酶IIα、胞浆动力蛋白重链和微管相关蛋白2，而不是PSD-95或AMPA受体，以Gd3+敏感的方式从大多数树突棘中释放出来。进一步发现，冷诱导的微管蛋白从树突棘上退出是一个能量依赖的过程，涉及Gd3+敏感的钙通道和兰尼定受体的激活。这些结果表明，温度、钙离子浓度和神经元周围介质能量供应的变化会影响树突棘的蛋白质组成，并可能影响树突棘细胞质中亚细胞组织的蛋白质组成，如突触后密度。(C)2008年Wiley-Liss，Inc.
Dendritic spines are small protrusions on neuronal dendrites and the major target of the excitatory inputs in mammalian brains. Cultured neurons and brain slices are important tools in studying the biochemical and cellular properties of dendritic spines. During the processes of immunocytochemical studies of neurons and the preparation of brain slices, neurons were often kept at temperatures lower than 37 degrees C for varied lengths of time. This study sought to investigate whether and how cold treatment would affect the protein composition of dendritic spines. The results indicated that upon cold treatment four postsynaptic proteins, namely, alpha,beta-tubulins, calcium, calmodulin-dependent protein kinase II alpha, and cytoplasmic dynein heavy chain and microtubule-associated protein 2, but not PSD-95 or AMPA receptors, exited from the majority of dendritic spines of cultured rat hippocampal neurons in a Gd3+-sensitive manner. The cold-induced exit of tubulins from dendritic spines was further found to be an energy-dependent process involving the activation of Gd3+-sensitive calcium channels and ryanodine receptors. The results thus indicate that changes in temperature, calcium concentration, and energy supply of the medium surrounding neurons would affect the protein composition of the dendritic spines and conceivably the protein composition of the subcellular organizations, such as the postsynaptic density, in the cytoplasm of dendritic spines. (c) 2008 Wiley-Liss, Inc.