Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.
Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.
复制标题
通过与酪蛋白络合从金橘中提取的 β-隐黄质，增强 Caco-2 细胞单层的水分散性和渗透性。
DOI：
10.1016/j.foodchem.2021.129822
复制
发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
N. Eto
中科院分区：
文献类型：
--
作者：
T. Ohima;K. Takanashi;A. Inada;M. Yamasaki;Y. Yamasaki;N. Eto
β-隐黄质（BCX）具有潜在的治疗和健康益处。然而，由于BCX的水溶性较差，其吸收率较低。本研究制备了BCX与酪蛋白（Cas）的配合物，以提高BCX的水分散性和生物利用度。通过固液萃取和皂化，从冻干金桔粉中定量回收BCX。该络合作用显著提高了BCX在酸性和中性条件下的表观溶解度。使用Caco-2细胞单层进行细胞膜渗透试验，以评估BCX-Cas复合物的生物利用度。体外消化该复合物和空白样品，并将其添加到Caco-2细胞膜的顶端。24 h后使用BCX - cas配合物的BCX渗透量是空白的22.7倍。因此，BCX与Cas的络合极大地提高了金桔提取物中BCX的生物利用度。
β-Cryptoxanthin (BCX) possesses potential therapeutic and health benefits. However, BCX absorption is low because of its poor aqueous solubility. In this study, a complex between BCX and casein (Cas) was prepared to improve the water dispersibility and bioavailability of BCX. BCX was recovered quantitatively from freeze-dried kumquat powder through solid–liquid extraction and saponification. The complexation significantly improved the apparent solubility of BCX under acidic and neutral conditions. A cell membrane permeation test using a Caco-2 cell monolayer was performed to evaluate the bioavailability of the BCX–Cas complex. This complex and a blank sample were digestedin vitroand added to the apical side of the Caco-2 cell membrane. The quantity of BCX that permeated using the BCX–Cas complex after 24 h was 22.7 times greater than that of the blank. Thus, complexation of BCX with Cas improved dramatically the bioavailability of BCX from a kumquat extract.