Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar
Possible functional involvement of thymosin beta 4 in developing tooth germ of mouse lower first molar
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DOI：
10.1007/s00418-005-0040-x
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发表时间：
2005-09-01
影响因子：
2.3
通讯作者：
Sakai, H
中科院分区：
文献类型：
--
作者：
Akhter, M;Kobayashi, I;Sakai, H
我们研究了胸腺素β4(Tβ4)在发育中的小鼠下颌第一磨牙中的详细原位表达模式。在胚胎第10.5天(E10.5)和增厚的牙齿上皮细胞中，胚胎10.5天(E10.5)和胚胎12天(E12)的牙齿上皮细胞均有Tβ4mRNA的表达。E13的凹陷性上皮芽、E14和E14.5的成釉器官、E14.5的初级釉质结(PEK)均有原位信号。在E15和E15.5处，该信号定位于釉器外层的上皮细胞。在这三个阶段的PEK中没有发现信号。胚胎16、17的内釉上皮、颈环和牙板均有Tβ4基因的表达。在胎龄18天、出生后第1天(N1)和出生后第1天(N1)和出生后第1天(N2)，Tβ4mRNA在极化的内上皮细胞中均有表达。但在N3时，信号强度明显减弱。本文首次报道了Tβ4在牙胚发育过程中的表达，并可能在牙胚的启动、生长和分化中发挥作用。
We examined the detailed in situ expression pattern of thymosin beta 4 (T beta 4) in the developing mouse mandibular first molar. T beta 4 mRNA was expressed in the presumptive dental epithelium at embryonic day 10.5 (E10.5) and in the thickened dental epithelium at E12. An in situ signal was observed in the invaginated epithelial bud at E13, in the enamel organ at E14 and E14.5, and in the primary enamel knot (PEK) at E14.5. The signal was localized in the epithelial cells of the outer layer of the enamel organ at E 15 and E 15.5. No signal was found in the PEK at these stages. T beta 4 mRNA was expressed in the inner enamel epithelium, cervical loop and dental lamina at E16 and E17. The expression of T beta 4 mRNA was observed in the polarized inner epithelial cells at E18, newborn day 1 (N1) and N2. However, the signal intensity decreased markedly at N3. We herein report for the first time that T beta 4 is distinctly expressed in developing tooth germ, and it may also play functional roles in the initiation, growth and differentiation of tooth germ.