Under the rug: Abandoning persistent misconceptions that obfuscate organelle evolution
Under the rug: Abandoning persistent misconceptions that obfuscate organelle evolution
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DOI：
10.1016/j.ympev.2020.106903
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发表时间：
2020-10-01
影响因子：
4.1
通讯作者：
Mandel, Jennifer R.
中科院分区：
文献类型：
--
作者：
Goncalves, Deise J. P.;Jansen, Robert K.;Mandel, Jennifer R.
在过去的二十年中，下一代测序的出现和发展使得积累大量的序列读数成为可能，这些序列读数可用于组装完整或接近完整的细胞器基因组（质体组或有丝分裂基因组）。结果是细胞器基因组序列的可用性爆炸性增加，目前在GenBank上可获得超过4000种不同的绿色植物。在同一时期，植物分子生物学家大大提高了对细胞器DNA的结构、修复、复制、重组、转录和翻译以及遗传的理解。不幸的是，许多植物进化生物学家不知道或忽略了这方面的知识，导致错误的几个现象，是关键的系统发育和进化研究使用细胞器基因组。我们相信，面对这些关于细胞器基因组组织，组成和遗传的误解，将提高我们对细胞器进化的进化过程的理解。在这里，我们讨论了四个误解，可以限制进化生物学的研究，并导致不准确的遗传学和不正确的结构的细胞器DNA用于推断细胞器的进化。
The advent and advance of next generation sequencing over the past two decades made it possible to accumulate large quantities of sequence reads that could be used to assemble complete or nearly complete organelle genomes (plastome or mitogenome). The result has been an explosive increase in the availability of organelle genome sequences with over 4000 different species of green plants currently available on GenBank. During the same time period, plant molecular biologists greatly enhanced the understanding of the structure, repair, replication, recombination, transcription and translation, and inheritance of organelle DNA. Unfortunately many plant evolutionary biologists are unaware of or have overlooked this knowledge, resulting in misrepresentation of several phenomena that are critical for phylogenetic and evolutionary studies using organelle genomes. We believe that confronting these misconceptions about organelle genome organization, composition, and inheritance will improve our understanding of the evolutionary processes that underly organelle evolution. Here we discuss four misconceptions that can limit evolutionary biology studies and lead to inaccurate phylogenies and incorrect structure of the organellar DNA used to infer organelle evolution.