Mammalian SWI/SNF Chromatin Remodeling Complexes in Embryonic Stem Cells: Regulating the Balance Between Pluripotency and Differentiation.
Mammalian SWI/SNF Chromatin Remodeling Complexes in Embryonic Stem Cells: Regulating the Balance Between Pluripotency and Differentiation.
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DOI：
10.3389/fcell.2020.626383
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发表时间：
2020
影响因子：
5.5
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Ye Y;Chen X;Zhang W
胚胎干细胞（ESCs）维持和调节自我更新和多谱系细胞分化之间的平衡的独特能力，对所有发育过程的完整性做出了不可或缺的贡献，导致了一个有机体在其成体形式的出现。ESC的命运决定是倾向于自我更新还是分化为特定的细胞系，很大程度上取决于通过基因表达调控的转录组调节。染色质重塑复合体在促进染色质结构变化中发挥重要作用，从而导致基因表达变化，这是控制多能性和分化之间平衡的ESC命运选择的关键。BAF （Brg/ brahma相关因子）或哺乳动物SWI/SNF复合物利用ATP水解产生的能量改变染色质状态，从而控制转录调节因子的可及性，最终影响转录组和细胞命运。有趣的是，尽管BAF复合物影响ESC命运选择的确切分子机制仍未被充分探索，但最近的遗传学研究已通过ESCs中各种BAF组分的基因表达调节显示了BAF复合物在ESCs自我更新和分化中的需求。本文综述了BAF复合物对ESC功能的影响，重点介绍了BAF复合物对ESC多能性和分化平衡的调节作用。将重点讨论ESC生物学中BAF遗传需求的机制基础，以及其与ESC中关键转录因子或其他染色质重塑因子相互作用的结果。
The unique capability of embryonic stem cells (ESCs) to maintain and adjust the equilibrium between self-renewal and multi-lineage cellular differentiation contributes indispensably to the integrity of all developmental processes, leading to the advent of an organism in its adult form. The ESC fate decision to favor self-renewal or differentiation into specific cellular lineages largely depends on transcriptome modulations through gene expression regulations. Chromatin remodeling complexes play instrumental roles to promote chromatin structural changes resulting in gene expression changes that are key to the ESC fate choices governing the equilibrium between pluripotency and differentiation. BAF (Brg/Brahma-associated factors) or mammalian SWI/SNF complexes employ energy generated by ATP hydrolysis to change chromatin states, thereby governing the accessibility of transcriptional regulators that ultimately affect transcriptome and cell fate. Interestingly, the requirement of BAF complex in self-renewal and differentiation of ESCs has been recently shown by genetic studies through gene expression modulations of various BAF components in ESCs, although the precise molecular mechanisms by which BAF complex influences ESC fate choice remain largely underexplored. This review surveys these recent progresses of BAF complex on ESC functions, with a focus on its role of conditioning the pluripotency and differentiation balance of ESCs. A discussion of the mechanistic bases underlying the genetic requirements for BAF in ESC biology as well as the outcomes of its interplays with key transcription factors or other chromatin remodelers in ESCs will be highlighted.
影响因子：
5.9
作者：
Weidgang, Clair E.;Russell, Ronan;Tata, Purushothama R.;Kuehl, Susanne J.;Illing, Anett;Mueller, Martin;Lin, Qiong;Brunner, Cornelia;Boeckers, Tobias M.;Bauer, Kerstin;Kartikasari, Apriliana E. R.;Guo, Yanchun;Radenz, Melanie;Bernemann, Christof;Weiss, Matthias;Seufferlein, Thomas;Zenke, Martin;Iacovino, Michelina;Kyba, Michael;Schoeler, Hans R.;Kuehl, Michael;Liebau, Stefan;Kleger, Alexander
通讯作者：
Kleger, Alexander