Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator
Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator
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DOI：
10.1111/j.1365-2672.2005.02686.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Chang, J
中科院分区：
文献类型：
--
作者：
Rogers, JV;Sabourin, CLK;Chang, J
目的：研究过氧化氢气体对炭疽杆菌、枯草芽孢杆菌和嗜热脂肪土芽孢杆菌孢子在室内表面材料上的去污效果。subtilis和G.将嗜热脂肪芽孢杆菌孢子在7种类型的室内表面上干燥，并暴露于>= 1000 ppm的过氧化氢气体20 min。过氧化氢暴露显著降低了活B。anthracis，B. B. subtilis和G.嗜热脂肪芽孢杆菌孢子在除G.嗜热脂肪菌在工业地毯上当比较B的活孢子减少时，观察到显著差异。炭疽病的症状同时评价了气态过氧化氢对生物指示剂和孢子条生长的有效性，作为去污的定性评估。在1和7天postexasthenes，净化生物指示剂和孢子条没有表现出增长，而nondeconpatinated样品显示growth.Conclusions：显着差异，在多孔和无孔表面上的过氧化氢气体的去污效力进行了观察时，比较平均对数减少B。炭疽孢子与B. subtilis和G.研究的意义和影响：这些结果为B的去污提供了比较信息。用过氧化氢气体将炭疽孢子与替代物放在室内表面上。
Aims: To evaluate the decontamination of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surface materials using hydrogen peroxide gas.Methods and Results: Bacillus anthracis, B. subtilis, and G. stearothermophilus spores were dried on seven types of indoor surfaces and exposed to >= 1000 ppm hydrogen peroxide gas for 20 min. Hydrogen peroxide exposure significantly decreased viable B. anthracis, B. subtilis, and G. stearothermophilus spores on all test materials except G. stearothermophilus on industrial carpet. Significant differences were observed when comparing the reduction in viable spores of B. anthracis with both surrogates. The effectiveness of gaseous hydrogen peroxide on the growth of biological indicators and spore strips was evaluated in parallel as a qualitative assessment of decontamination. At 1 and 7 days postexposure, decontaminated biological indicators and spore strips exhibited no growth, while the nondecontaminated samples displayed growth.Conclusions: Significant differences in decontamination efficacy of hydrogen peroxide gas on porous and nonporous surfaces were observed when comparing the mean log reduction in B. anthracis spores with B. subtilis and G. stearothermophilus spores.Significance and Impact of the Study: These results provide comparative information for the decontamination of B. anthracis spores with surrogates on indoor surfaces using hydrogen peroxide gas.