Nephritogenicity of proteoglycans. III. Mechanism of immune deposit formation.
Nephritogenicity of proteoglycans. III. Mechanism of immune deposit formation.
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蛋白多糖的肾炎性。
DOI：
10.1038/ki.1988.166
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发表时间：
1988
影响因子：
19.6
通讯作者：
Kanwar,YS
中科院分区：
文献类型：
--
作者：
Makino,H;Lelongt,B;Kanwar,YS
蛋白多糖的致肾性。三.免疫存款形成机制。针对肾小球基底膜（GBM）硫酸乙酰肝素-蛋白聚糖的抗体给药至致敏大鼠导致上皮下免疫复合物沉积诱导膜性肾病。在这项研究中，我们研究了负责在抗HS-PG模型中形成上皮下免疫复合物沉积的机制。在最初的实验中，观察到静脉内给药的放射性碘标记抗体仅定位于肾小球毛细血管壁的区域，在该区域观察到上皮下沉积物。为了确定它们在上皮下空间中的唯一定位，研究了静脉内施用的抗体的运动动力学。抗体在施用后几分钟内定位于GBM的内层。它在几个小时内在GBM的内层和外层达到平衡。然后，24小时后，它逐渐从GBM的内层消失，仅存在于外层。抗体从内层的快速清除可能与GBM内横向固有血浆流体电流的动力学差异有关。持久性的异源抗体专门在外层和宿主自体抗体的可用性可能导致免疫复合物沉积在上皮下空间的发展。缺乏血浆水电流的肾小球显示GBM或系膜基质的内层和外层中的抗体浓度没有变化。此外，未观察到抗体与足突或内脏上皮细胞的质膜结合。这些数据表明，肾小球毛细血管壁的生物化学-生物物理特性，与其肾小球内血流动力学一致，很可能在该模型中上皮下免疫复合物沉积的发展中发挥了重要作用。
Nephritogenicity of proteoglycans. III. Mechanism of immune deposit formation. Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepithelial immune-complex deposits in the anti-HS-PG model. In initial experiments, the intravenously administered radioiodinated antibody was seen exclusively localized in the regions of the glomerular capillary wall where the subepithelial deposits were observed. To determine their exclusive localization in the subepithelial space, kinetics of movement of the intravenously administered antibody was investigated. The antibody localized in the inner layers of the GBM within a few minutes after its administration. It equilibrated in the inner and outer layers of the GBM in a matter of a few hours. Then, after 24 hours, it gradually disappeared from the inner layers of the GBM and persisted in the outer layers only. The ready clearance of the antibody from the inner layers may be related to the differential in the kinetics of lateral intrinsic plasma fluid currents within the GBM. The persistence of heterologous antibody exclusively in the outer layers and the availability of host autologous antibodies probably resulted in the development of immune complex deposits in the subepithelial space. The glomeruli devoid of plasma water currents showed no change in the concentration of the antibody in the inner and outer layers of the GBM or mesangial matrix. Also, no antibody binding was observed with the plasmalemma of either the foot processes or visceral epithelia. The data suggest that the biochemical-biophysical properties of the glomerular capillary wall, in concert with its intraglomerular hemodynamics, most likely played a significant role in the development of subepithelial immune-complex deposits in this model.
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肾小球滤过和蛋白尿的生物生理学。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Kanwar,YS
通讯作者：
Kanwar,YS
硫酸化糖胺聚糖在肾小球基底膜和系膜基质中的分布。
影响因子：
6.6
作者：
Kanwar,YS;Jakubowski,ML;Rosenzweig,LJ
通讯作者：
Rosenzweig,LJ
影响因子：
10.5
作者：
M. Mannik;V. Gauthier
通讯作者：
V. Gauthier
支气管脂肪萎缩性胰腺炎
影响因子：
5
作者：
L. Burkhardt
通讯作者：
L. Burkhardt
阳离子化抗原与大鼠肾小球基底膜的相互作用：原位免疫复合物形成。
影响因子：
19.6
作者：
A. Vogt;Rolf Rohrbach;F. Shimizu;H. Takamiya;S. Batsford
通讯作者：
S. Batsford