Local Administration of Glial Cell Line-derived Neurotrophic Factor Improves Behavioral and Histological Deficit of Neonatal Erb’s Palsy in Rats
Local Administration of Glial Cell Line-derived Neurotrophic Factor Improves Behavioral and Histological Deficit of Neonatal Erb’s Palsy in Rats
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DOI：
10.1227/01.neu.0000083029.91562.7f
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发表时间：
2003-10
期刊：
影响因子：
4.8
通讯作者：
H. Ochiai;T. Ikeda;K. Mishima;Naoya Aoo;K. Iwasaki;M. Fujiwara;S. Nakano;T. Ikenoue;S. Wakisaka
中科院分区：
文献类型：
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作者：
H. Ochiai;T. Ikeda;K. Mishima;Naoya Aoo;K. Iwasaki;M. Fujiwara;S. Nakano;T. Ikenoue;S. Wakisaka
目的从行为学和组织学角度评价局部给予神经胶质细胞系源性神经营养因子（GDNF）对新生大鼠神经节前神经麻痹的影响。方法采用横断7日龄大鼠左C5-C7神经前后根的方法建立Erb麻痹模型。将大鼠分为gdnf处理组（n = 10）和载药组（n = 11）。横切根后，保持横切神经近端和远端残端接触，横切点和横切根的整个椎内部分用10g GDNF或生理盐水浸泡的明胶泡沫塑料包裹。行为评估包括足部缺陷测试和前爪肌肉力量测试，所有这些都是在手术后的第3至第7周进行的。术后7周处死各组大鼠，在C5-C7处计数前角细胞数，比较各侧差异。结果车辆处理组足部断层检查显示根横断侧前肢功能异常。然而，在gdnf治疗组中，根据术后第3至第6周获得的足断层测试结果，观察到前肢功能有明显改善。在组织学评估中，车辆治疗组手术一侧的前角细胞数量明显少于对侧各节段的前角细胞数量。然而，在gdnf处理组中，无论从哪一边提取，任何部分都没有差异。结论在新生儿神经节前Erb麻痹模型中局部给予GDNF可显著改善其行为学和组织学上的缺陷。
OBJECTIVEWe sought to evaluate from a behavioral and histological viewpoint the effect of local administration of glial cell line-derived neurotrophic factor (GDNF) on neonatal preganglionic Erb’s palsy in rats. METHODSThe Erb’s palsy model was produced by transecting the anterior and posterior roots of the left C5–C7 nerves of 7-day-old rats. The rats were divided into GDNF-treated (n = 10) and vehicle-treated groups (n = 11). After we transected the roots, contact in the proximal and distal stumps of the transected nerves was maintained, and the transected point and the entire intraspinal portion of the transected roots were enclosed by Gelfoam soaked with 10 g GDNF or saline. The behavioral evaluation consisted of a foot-fault test and a forepaw muscle strength test, all of which were performed from the third to the seventh weeks after the operation. Seven weeks after the operation, all rats were killed, the number of anterior horn cells was counted at C5–C7, and the differences on each side were compared. RESULTSIn the vehicle-treated group, the foot-fault test indicated an abnormality in forelimb function on the root transection side. In the GDNF-treated group, however, significant improvement in forelimb function was observed on the basis of the foot-fault test results obtained in the third to sixth weeks after the operation. In the histological evaluation, the number of anterior horn cells from the side in which the operation took place in the vehicle-treated group was significantly less than that taken from the contralateral side at each segment. In the GDNF-treated group, however, there was no difference in any of the segments, regardless of the side from which they were taken. CONCLUSIONLocal administration of GDNF in a neonatal preganglionic Erb’s palsy model resulted in significant improvement in deficits on the basis of behavioral and histological evaluations.