MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfection
MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfection
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DOI：
10.1016/j.biocel.2004.04.024
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Ahn, C
中科院分区：
文献类型：
--
作者：
Kim, JY;Kim, D;Ahn, C
Due to their unique capacity to self-renew and for multiple differentiation, stem cells are considered promising candidates for cell replacement therapy in many devastating diseases. However, studies on immune rejection, which is a major problem facing Successful stein cell therapy, are rare. In this study, we examined MHC expression in the M13SV1 cell line, which has previously been shown to have stem cell properties and to be non-tu mori genic, in order to determine whether human adult stem cells might be rejected after transplantation. Our results show low expression levels of MHC class I molecules on the surface of these cells. An induction of MHC class I expression was observed when the cells were treated with IFN-gamma. Maximal induction of MHC class protein expression was observed at 48 h after treatment with concentrations above 5 ng/ml of IFN-gamma. Elevated MHC class I levels were Sustained for 72 It after withdrawing IFN-gamma. Therefore, we introduced human cytomegalovirus (hCMV) US genes, which are known to be able to reduce MHC class I expression on the cell surface after infection, into M13SV1 cells. Cells transfected with the hCMV US2, US3, US6 or US11 genes exhibited a reduction (40-60%) of MHC class I expression compared with mock-transfected cells. These results suggest that human adult stem cells are capable of expressing high levels of MHC class I proteins, and thus may be rejected on transplantation unless they are modified. In addition, viral stealth mechanisms can be exploited for stein cell transplantation. (C) 2004 Elsevier Ltd. All rights reserved.
