Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling
Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling
复制标题
DOI：
10.1016/j.bbrc.2005.12.053
复制
发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Wakisaka, S
中科院分区：
文献类型：
--
作者：
Harada, H;Ichimori, Y;Wakisaka, S
在牙齿发育过程中，中间层在成釉细胞层的近侧以扁平的细胞层的形式发育。然而，关于起源和作用的信息很少。在本研究中，我们用DiI作为示踪剂，表明在大鼠切牙器官培养中，一些中间层细胞起源于内釉质上皮(IEE)。免疫组织化学和原位杂交研究表明，中间层细胞表达Notch1蛋白和Hes1mRNAs，而IEE和成釉细胞表达Jagged1。此外，我们使用牙齿上皮细胞系HAT-7研究了Notch信号的作用。重组Jagged1蛋白可促进HAT-7细胞中间层细胞的出现，而抗Jagged1抗体中和可抑制这种作用。此外，Notch1内部结构域的过表达增加了中间层细胞的数量。我们假设中间层系通过Notch信号与成釉细胞系相区别。(C)2005 Elsevier Inc.保留所有权利。
The stratum intermedium develops as flattened cell layers on the proximal side of the ameloblast layer during tooth development. However, little information is available regarding the origin and the role. In this study, we indicate that some stratum intermedium cells originate from the inner enamel epithelium (IEE) in rat incisor organ cultures using DiI as a tracer. Immunohistochemical and in situ hybridization studies showed that the stratum intermedium cells express the Notch1 protein and Hes1 mRNAs, while the IEE and ameloblasts express the Jagged1. Further, we examined the role of Notch signaling using the dental epithelial cell line HAT-7. Recombinant Jagged1 protein enhanced the appearance of stratum intermedium cells in HAT-7 cultures and neutralization with an anti-Jagged1 antibody inhibited these effects. Additionally, overexpression of the Notch1 internal domain increased the number of stratum intermedium cells. We hypothesize that the stratum intermedium lineage differentiates from the ameloblast lineage via Notch signaling. (c) 2005 Elsevier Inc. All rights reserved.