Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring
Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring
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DOI：
10.1016/j.mimet.2020.105858
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发表时间：
2020-03-01
影响因子：
2.2
通讯作者：
Shome, Rajeswari
中科院分区：
文献类型：
--
作者：
Kalleshamurthy, Triveni;Skariah, Somy;Shome, Rajeswari
布鲁氏菌病是一种重要的人畜共患病，由布鲁氏菌属细菌引起，构成严重的公共卫生危害。在本研究中，两种用于布鲁氏菌病诊断的高度特异性血清学检测荧光偏振测定（FPA）和竞争性ELISA（cELISA）在实验室标准化，并与玫瑰红平板试验（RBPT）、间接ELISA（iELISA）和商业cELISA试剂盒进行评估和比较。为了进行测试评估，分析了 1386 份血清样本 [明显健康的动物 (n = 260)、来自布鲁氏菌感染农场的样本 (n = 701) 和流产布鲁氏菌 S19 接种疫苗的动物 (n = 425)]，以评估在接种流产布鲁氏菌 S19 后的牛群中进行适当的诊断测试。在表面上健康的无布鲁氏菌病农场中，发现 RBPT、iELISA、内部 FPA 和 cELISA 比商业 cELISA 具有更高的特异性。在从受感染农场收集的样本中，商业 cELISA 试剂盒比其他血清学测试更敏感。 FPA 显示的灵敏度几乎与 RBPT 相同，并且在受感染的农场中，内部 cELISA 显示出比 RBPT 更高的灵敏度。在具有持久性疫苗抗体的动物中，只有内部 FPA 和 cELISA 的特异性较高，分别为 87.64% 和 90.27%。其他测试 RBPT 和 iELISA 显示与疫苗抗体的反应性与感染抗体的反应性相似，而商业 cELISA 试剂盒显示出 47.69% 的中等特异性。根据这些发现，建议使用 RBPT、iELISA 和 cELISA 来筛查受感染的牛群，并且内部开发的具有经过验证的特异性的 FPA 和 cELISA 测试可用于对流产布鲁氏菌 S19 接种后动物群体中的布鲁氏菌病进行确诊。
Brucellosis is an important zoonosis that constitutes a serious public health hazard which is caused by a bacterium belonging to the genus Brucella. In the present study, two highly specific serological tests for brucellosis diagnosis, fluorescence polarization assay (FPA) and competitive ELISA (cELISA) were standardized in the laboratory, evaluated and compared with rose bengal plate test (RBPT), indirect ELISA (iELISA) and commercial cELISA kit. For test evaluation, 1386 serum samples [apparently healthy animals (n = 260), samples from Brucella infected farms (n = 701) and B. abortus S19 vaccinated animals (n = 425)] were analyzed to assess suitable diagnostic test in B. abortus S19 post vaccinated bovine population. In apparently healthy brucellosis free farms, RBPT, iELISA, in-house FPA and cELISA were found to be highly specific than commercial cELISA. Commercial cELISA kit was comparatively more sensitive than other serological tests in samples collected from infected farms. The FPA showed sensitivity nearly equal to RBPT and in-house cELISA showed greater sensitivity than RBPT in infected farms. In animals with persistent vaccinal antibodies, only in-house FPA and cELISA recorded higher specificity of 87.64 and 90.27%, respectively. The other tests, RBPT and iELISA displayed similar reactivity with vaccine antibodies to that of infection antibodies whereas commercial cELISA kit showed an intermediate specificity of 47.69%. With these findings, RBPT, iELISA and cELISA are suggested for screening infected herds, and in-house developed FPA and cELISA tests with a proven specificity can be used for confirmatory diagnosis of brucellosis in B. abortus S19 post vaccinated animal populations.