Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii.
Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii.
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石松植物卷柏中八种微生物型萜烯合成酶的生化特征和功能
DOI：
10.3390/ijms22020605
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发表时间：
2021-01-09
影响因子：
5.6
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Zhao Y;Hu T;Liu R;Hao Z;Liang G;Li G
Selaginella moellendorffii is a lycophyte, a member of an ancient vascular plant lineage. Two distinct types of terpene synthase (TPS) genes were identified from this species, including S. moellendorffii TPS genes (SmTPSs) and S. moellendorffii microbial TPS-like genes (SmMTPSLs). The goal of this study was to investigate the biochemical functions of SmMTPSLs. Here, eight full-length SmMTPSL genes (SmMTPSL5, -15, -19, -23, -33, -37, -46, and -47) were functionally characterized from S. moellendorffii. Escherichia coli-expressed recombinant SmMTPSLs were tested for monoterpenes synthase and sesquiterpenes synthase activities. These enzymatic products were typical monoterpenes and sesquiterpenes that have been previous shown to be generated by typical plant TPSs when provided with geranyl diphosphate (GPP) and farnesyl diphosphate (FPP) as the substrates. Meanwhile, SmMTPSL23, -33, and -37 were up-regulated when induced by alamethicin (ALA) and methyl jasmonate (MeJA), suggesting a role for these genes in plants response to abiotic stresses. Furthermore, this study pointed out that the terpenoids products of SmMTPSL23, -33, and -37 have an antibacterial effect on Pseudomonas syringae pv. tomato DC3000 and Staphylococcus aureus. Taken together, these results provide more information about the catalytic and biochemical function of SmMTPSLs in S. moellendorffii plants.
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影响因子：
2.4
作者：
ONAWUNMI, GO
通讯作者：
ONAWUNMI, GO
影响因子：
4.1
作者：
MOLEYAR, V;NARASIMHAM, P
通讯作者：
NARASIMHAM, P
影响因子：
11.6
作者：
Kumar, Santosh;Kempinski, Chase;Chappell, Joe
通讯作者：
Chappell, Joe
影响因子：
2
作者：
MORRIS, JA;KHETTRY, A;SEITZ, EW
通讯作者：
SEITZ, EW
DOI：
10.1073/pnas.1204300109
发表时间：
2012-09-04
影响因子：
11.1
作者：
Li, Guanglin;Koellner, Tobias G.;Chen, Feng
通讯作者：
Chen, Feng