Immunohistochemical localization of dynorphin (1-8) in hypothalamic magnocellular neurons: evidence for absence of proenkephalin.
Immunohistochemical localization of dynorphin (1-8) in hypothalamic magnocellular neurons: evidence for absence of proenkephalin.
复制标题
下丘脑大细胞神经元中强啡肽 (1-8) 的免疫组织化学定位：脑啡肽原缺失的证据。
DOI：
10.1016/0024-3205(82)90204-1
复制
发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Barchas,JD
中科院分区：
文献类型：
--
作者：
Weber,E;Roth,KA;Evans,CJ;Chang,JK;Barchas,JD
在大鼠下丘脑大细胞神经元内，强啡肽（1-8）免疫反应呈荧光染色。在大细胞神经元中未检测到免疫反应性甲硫氨酸脑啡肽-Arg 6-Gly 7-Leu 8，这是肾上腺髓质前脑啡肽分子的片段。然而，一个强大的甲硫氨酸-脑啡肽-Arg 6-Gly 7-Leu 8-样免疫染色被认为是在大脑的其他区域。这些结果表明，在大细胞神经元中强啡肽（1-8）独立于脑啡肽原而存在，因而大细胞神经元代表了脑内第三类阿片肽神经元系统。然而，这些观察结果并不排除脑啡肽原和强啡肽相关肽在大脑其他区域共存的可能性。
Dynorphin(1–8) immunoreactivity was visualized by immunohistofluorescence in hypothalamic magnocellular neurons of the rat. No immunoreactive met-enkephalin-Arg6-Gly7-Leu8, a fragment of the adrenal medulla pro-enkephal in molecule, was detected in magnocelullar neurons. However, a strong met-enkephalin-Arg6-Gly7-Leu8-like immunostaining was seen in other regions of the brain. These results suggest that in magnocellular neurons dynorphin(1–8) exists independently from pro-enkephalin and therefore the magnocellular neurons represent a third opioid peptide neuronal system in brain. These observations, however, do not rule out a coexistence of proenkephalin and dynorphin-related peptides in other regions of the brain.