Community-wide transcriptome of the oral microbiome in subjects with and without periodontitis
Community-wide transcriptome of the oral microbiome in subjects with and without periodontitis
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DOI：
10.1038/ismej.2014.23
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发表时间：
2014-08-01
期刊：
影响因子：
11
通讯作者：
Frias-Lopez, Jorge
中科院分区：
文献类型：
--
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
尽管对人类微生物组的系统发育组成的了解不断增加，但我们对群落中生物体的原位活动及其彼此之间以及与环境的相互作用的了解仍然有限。表征人类微生物组的基因表达谱对于联系细菌群落不同成员在健康和疾病中的作用至关重要。口腔微生物群是人体中最复杂的微生物群落之一，在某些尚未完全了解的情况下，健康的微生物群落会经历向致病状态的转变，从而引起牙周炎（一种多微生物炎症性疾病）。我们在这里报告了六名牙周健康个体和七名牙周炎个体的龈下微生物组的原位全基因组转录组。代谢活动的总体情况表明，铁的获取、脂多糖合成和鞭毛合成是定义疾病的主要活动。出乎意料的是，牙周炎受试者中上调的绝大多数毒力因子来自不被认为是主要牙周病原体的生物体。其基因表达谱被表征的生物体之一是未培养的候选部门TM7，显示患病群落中假定的毒力因子的上调。这些数据增强了对牙周病特征的核心活动以及龈下群落中个体生物体在牙周炎中所起作用的理解。
Despite increasing knowledge on phylogenetic composition of the human microbiome, our understanding of the in situ activities of the organisms in the community and their interactions with each other and with the environment remains limited. Characterizing gene expression profiles of the human microbiome is essential for linking the role of different members of the bacterial communities in health and disease. The oral microbiome is one of the most complex microbial communities in the human body and under certain circumstances, not completely understood, the healthy microbial community undergoes a transformation toward a pathogenic state that gives rise to periodontitis, a polymicrobial inflammatory disease. We report here the in situ genome-wide transcriptome of the subgingival microbiome in six periodontally healthy individuals and seven individuals with periodontitis. The overall picture of metabolic activities showed that iron acquisition, lipopolysaccharide synthesis and flagellar synthesis were major activities defining disease. Unexpectedly, the vast majority of virulence factors upregulated in subjects with periodontitis came from organisms that are not considered major periodontal pathogens. One of the organisms whose gene expression profile was characterized was the uncultured candidate division TM7, showing an upregulation of putative virulence factors in the diseased community. These data enhance understanding of the core activities that are characteristic of periodontal disease as well as the role that individual organisms in the subgingival community play in periodontitis.