Desulfoplanes formicivorans gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a brackish meromictic lake, and emended description of the family Desulfomicrobiaceae
Desulfoplanes formicivorans gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a brackish meromictic lake, and emended description of the family Desulfomicrobiaceae
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食蚁兽脱磺菌属
DOI：
10.1099/ijsem.0.000484
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发表时间：
2015
影响因子：
2.8
通讯作者：
Hisaya Kojima and Manabu Fukui
中科院分区：
文献类型：
--
作者：
Miho Watanabe;Hisaya Kojima and Manabu Fukui
从日本 Harutori 湖沉积物中分离出一种新型硫酸盐还原细菌，命名为 Pf12BT 菌株。细胞呈弧状（1.0 × 3.0–4.0 μm）、能动且革兰氏染色阴性。对于生长来说，最适pH为7.0-7.5，最适温度为42-45°C。菌株Pf12BT使用硫酸盐、硫代硫酸盐和亚硫酸盐作为电子受体。基因组DNA的G+C含量为55.4 mol%。主要细胞脂肪酸为C16 : 0和C18 : 0，该菌株呈脱硫病毒蛋白阳性。基于16S rRNA基因的系统发育分析表明，该新菌株属于Delta变形菌纲脱硫弧菌目。最接近的亲缘关系是Desulfomicrobium baculatumDSM 4028T，它与它有91%的16S rRNA基因序列相似性。在系统发育和表型特征的基础上，提出了属于脱硫微生物科的一个新属的新种，Desulfoplanes formicivoransgen。十一月，SP。十一月模式菌株为Desulfoplanes formicivoransis Pf12BT( = NBRC 110391T= DSM 28890T)。
A novel sulfate-reducing bacterium, designated strain Pf12BT, was isolated from sediment of meromictic Lake Harutori in Japan. Cells were vibroid (1.0 × 3.0–4.0 μm), motile and Gram-stain-negative. For growth, the optimum pH was 7.0–7.5 and the optimum temperature was 42–45 °C. Strain Pf12BTused sulfate, thiosulfate and sulfite as electron acceptors. The G+C content of the genomic DNA was 55.4 mol%. Major cellular fatty acids were C16 : 0and C18 : 0. The strain was desulfoviridin-positive. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the orderDesulfovibrionalesin the classDeltaproteobacteria. The closest relative wasDesulfomicrobium baculatumDSM 4028Twith which it shared 91 % 16S rRNA gene sequence similarity. On the basis of phylogenetic and phenotypic characterization, a novel species of a new genus belonging to the familyDesulfomicrobiaceaeis proposed,Desulfoplanes formicivoransgen. nov., sp. nov. The type strain ofDesulfoplanes formicivoransis Pf12BT( = NBRC 110391T= DSM 28890T).