Xanthomonas oryzae pv. oryzae type III effector PthXo3(JXOV) suppresses innate immunity, induces susceptibility and binds to multiple targets in rice
Xanthomonas oryzae pv. oryzae type III effector PthXo3(JXOV) suppresses innate immunity, induces susceptibility and binds to multiple targets in rice
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米黄单胞菌 pv.
DOI：
10.1093/femsle/fny037
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发表时间：
2018
影响因子：
2.1
通讯作者：
Song Congfeng
中科院分区：
文献类型：
--
作者：
Li Rongmei;Wang Shuo;Sun Ronghua;He Xiang;Liu Yongting;Song Congfeng
由talgenes编码的转录激活因子样（Transcription activator-like， TAL）效应物被认为是水稻黄单胞菌（xanthomonas oryzaepv）使用的关键毒力策略。oryzae（Xoo）引起水稻的细菌性叶枯病。TAL效应因子PthXo3是在菲律宾菌株PXO61中鉴定出的主要毒力因子，它通过与启动子区域的效应结合元件（effector-binding element， EBE）结合，诱导易感基因ossweet14的表达。本研究还从xoochinese菌株OS198和Japanese菌株JXOV中鉴定并分离到了pthxo3同源基因，分别命名为aspthxo3os198和pthXo3JXOV。将pthxo3jxovv注入PXO99A后，得到的菌株PXO99A/ pthxo3jxovv在23个水稻品种中有18个品种的毒力显著增加，其中最显著的是在水稻IRBB13中损伤长度和细菌繁殖增加。pthxo3jxov通过抑制超敏反应（hypersensitive response， HR）和胼胝质沉积来抑制植物的先天免疫。农杆菌介导的瞬时表达实验表明，除了ossweet14， pthxo3jxov还通过结合EBEs的启动子区域与其他靶标相互作用。我们的研究结果表明pthxo3jxov可能与多个靶点相互作用以执行其毒力功能。
Transcription activator-like (TAL) effectors encoded bytalgenes were recognized as a key virulence strategy used byXanthomonas oryzaepv.oryzae(Xoo) to cause bacterial leaf blight of rice. TAL effector PthXo3 is a major virulence factor identified in a PhilippineXoostrain PXO61, and it can induce the expression of susceptibility geneOsSWEET14by binding to the effector-binding element (EBE) in the promoter region. In this study,pthXo3homologous genes were also identified and isolated fromXooChinese strain OS198 and Japanese strain JXOV, which were named aspthXo3OS198and pthXo3JXOV, respectively. WhenpthXo3JXOVwas delivered into PXO99A, the resulting strain PXO99A/pthXo3JXOVsignificantly increased virulence in 18 out of 23 rice varieties tested, with the most prominent increase in lesion length and bacteria propagation in rice IRBB13. PthXo3JXOVsuppresses the plant's innate immunity by inhibiting hypersensitive response (HR) and callose deposition. TheAgrobacterium tumefaciens-mediated transient expression assays showed that, besidesOsSWEET14, PthXo3JXOValso interacts with other targets by binding to the EBEs in their promoter regions. Our results suggest that PthXo3JXOVmay interact with multiple targets to execute its virulence functions.