A GENETIC-MAP OF CITRUS BASED ON THE SEGREGATION OF ISOZYMES AND RFLPS IN AN INTERGENERIC CROSS
A GENETIC-MAP OF CITRUS BASED ON THE SEGREGATION OF ISOZYMES AND RFLPS IN AN INTERGENERIC CROSS
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DOI：
10.1007/bf00223980
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发表时间：
1992-06-01
影响因子：
5.4
通讯作者：
KUPPER, RS
中科院分区：
文献类型：
--
作者：
JARRELL, DC;ROOSE, ML;KUPPER, RS
同工酶和限制性片段长度多态性被用作标记在构建柑橘核基因组的遗传图谱。该图谱是基于两个属间杂种‘Sacaton’Citrumelo（Citrusparadisi Macf.）的60个后代的8个同工酶、1个蛋白质和37个RFLP位点的分离。x枳壳Poncirus trifoliata（L.）Raf.）和'Troyer' citrange（C. sinensis（L.）Osbeck x P. trifoliata），通常用作砧木。该图谱包含46个研究位点中的38个，分布在10个连锁群上。根据部分连锁数据估计基因组大小为1，700 cM。大约35%的基因组应该在10 cM内，58%在20 cM内的映射标记。3个连锁群中的8个位点和1个非连锁位点显著偏离孟德尔分离。
Isozymes and restriction fragment length polymorphisms were used as markers in the construction of a genetic map of the citrus nuclear genome. The map was based on the segregation of 8 isozyme, 1 protein, and 37 RFLP loci in 60 progeny of a cross of two intergeneric hybrids, 'Sacaton' citrumelo (Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.) and 'Troyer' citrange (C. sinensis (L.) Osbeck x P. trifoliata), often used as root-stocks. The map contains 38 of 46 studied loci distributed on ten linkage groups. A genome size of 1,700 cM was estimated from partial linkage data. Approximately 35% of the genome should be within 10 cM and 58% within 20 cM of the mapped markers. Eight loci in three linkage groups and 1 unlinked locus deviated significantly from Mendelian segregation.