Alcohol drinking produces brain region-selective changes in expression of inducible transcription factors
Alcohol drinking produces brain region-selective changes in expression of inducible transcription factors
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DOI：
10.1016/s0006-8993(99)02019-3
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发表时间：
1999-11-20
期刊：
影响因子：
2.9
通讯作者：
Ryabinin, AE
中科院分区：
文献类型：
--
作者：
Bachtell, RK;Wang, YM;Ryabinin, AE
绘制饮酒对神经活动的影响可以为酒精对行为的影响机制提供有价值的信息。本研究旨在确定饮酒对小鼠大脑中诱导转录因子（ITF）表达的影响。 C57BL/6J 小鼠经过训练，在 30 分钟的有限访问期内消耗 10% 乙醇/10% 蔗糖溶液。对照动物饮用 10% 蔗糖溶液或水。手术最后一天后，处死动物并对三种 ITF（c-Fos、FosB 和 Zif268）进行免疫组织化学分析。饮酒的动物大脑多个区域的 ITF 表达增加。具体而言，c-Fos 在伏隔核 (AcbC)、杏仁核中央核的内侧后腹侧部分 (CeMPV) 和 Edinger-Westphal 核 (EW) 中显着诱导。与食用蔗糖的动物相比，饮酒动物的齿状回中 c-Fos 的表达显着较低。然而，它与水对照没有显着差异。 AcbC、CeMPV 和 EW 中 c-Fos 的诱导与血液酒精浓度 (BAC) 显着相关。此外，与水对照相比，饮酒动物中 CeMPV 和 EW 中的 FosB 表达也显着更高。 EW 中的 FosB 表达与 BAG 显着相关。这些结果的意义是双重的。首先，我们的实验表明，ITF 映射是识别自愿饮酒后酒精引起的变化的有效策略。其次，他们提出了 AcbC、CeMPV 和 EW 中 ITF 表达与酒精中毒水平之间的关系。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Mapping the effects of alcohol consumption on neural activity could provide valuable information on mechanisms of alcohol's effects on behavior. The present study sought to identify effects of alcohol consumption on expression of inducible transcription factors (ITFs) in mouse brain. C57BL/6J mice were trained to consume 10% ethanol/10% sucrose solution during a 30-min limited access period. Control animals were given access to 10% sucrose solution or water. Following the final day of the procedure, animals were sacrificed and immunohistochemical analyses were performed for three ITFs (c-Fos, FosB, and Zif268). Alcohol-consuming animals had increased ITF expression in several brain areas. Specifically, c-Fos was significantly induced in the nucleus accumbens core (AcbC), the medial posteroventral portion of the central nucleus of the amygdala (CeMPV), and the Edinger-Westphal nucleus (EW), Expression of c-Fos was significantly lower in the dentate gyrus of alcohol-consuming animals vs. sucrose-consuming animals. However, it was not significantly different from the water controls. Induction of c-Fos in AcbC, CeMPV and EW was significantly related to blood alcohol concentrations (BAC). Furthermore, FosB expression in the CeMPV and the EW was also significantly higher in the alcohol-consuming animals vs. water controls. FosB expression in the EW was significantly related to BAG. The significance of these results is two-fold. First, our experiments demonstrate that ITF mapping is an effective strategy in identifying alcohol-induced changes following voluntary consumption. Second, they suggest a relationship between ITF expression in AcbC, CeMPV and EW and the level of alcohol intoxication. (C) 1999 Elsevier Science B.V. All rights reserved.