Comparison of endotoxin exposure assessment by bioaerosol impinger and filter-sampling methods
Comparison of endotoxin exposure assessment by bioaerosol impinger and filter-sampling methods
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DOI：
10.1128/aem.67.6.2775-2780.2001
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发表时间：
2001-06-01
影响因子：
4.4
通讯作者：
Cormier, Y
中科院分区：
文献类型：
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作者：
Duchaine, C;Thorne, PS;Cormier, Y
在猪舍和锯木厂的两项先前职业卫生研究中收集的环境评估数据允许使用空气采样过滤器和生物气溶胶撞击器对同时进行的三次平行内毒素测量进行比较。使用鲎试剂测定撞击器溶液和过滤器洗脱液中的内毒素浓度。在锯木厂，撞击器采样产生的内毒素浓度测量值显著高于使用IOM可吸入粉尘采样器的过滤器采样，且方差较低。重复测量的方差分析表明，这种关联仍然控制其他因素，如复制，锯木厂，锯木厂操作，木材类型，和相互作用的条款。猪舍中的内毒素浓度平均比锯木厂高10倍。这些样品证明撞击器和过滤器方法的内毒素浓度估计值相当，尽管使用撞击器方法的变异性较低。在这两种职业环境中，平行平行的复制品更均匀的撞击器样品比过滤器样品。本研究证明，撞击采样器采样是定量区域内毒素浓度的可接受方法。此外，当使用撞击采样器进行采样以定量空气微生物时，这些相同的撞击采样器溶液可以产生有效的内毒素暴露估计值，无需额外的过滤器采样。
Environmental assessment data collected in two prior occupational hygiene studies of swine barns and sawmills allowed the comparison of concurrent, triplicate, side-by-side endotoxin measurements using air sampling filters and bioaerosol impingers. Endotoxin concentrations in impinger solutions and filter eluates were assayed using the Limulus amebocyte lysate assay, In sawmills, impinger sampling yielded significantly higher endotoxin concentration measurements and lower variances than filter sampling with IOM inhalable dust samplers. Analysis of variance for repeated measures showed that this association remained after controlling for other factors such as replicate, sawmill, sawmill operation, wood type, and interaction terms. Endotoxin concentrations in the swine barns were 10-fold higher on average than in sawmills. These samples demonstrated comparable endotoxin concentration estimates for impinger and filter methods although the variability was Lower using the impinger method. In both occupational settings, side-by-side replicates were more uniform for the impinger samples than for the filter samples. This study demonstrates that impinger sampling is an acceptable method for quantitation of area endotoxin concentrations, Further, when sampling is performed with impingers for airborne microorganism quantitation, these same impinger solutions can yield valid endotoxin exposure estimates, negating the need for additional filter sampling.