Role of callose synthases in transfer cell wall development in tocopherol deficient Arabidopsis mutants.
Role of callose synthases in transfer cell wall development in tocopherol deficient Arabidopsis mutants.
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DOI：
10.3389/fpls.2014.00046
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发表时间：
2014
影响因子：
5.6
通讯作者：
Dellapenna D
中科院分区：
文献类型：
--
作者：
Maeda H;Song W;Sage T;Dellapenna D
生育酚(维生素E)是一种脂溶性抗氧化剂，由所有植物和藻类以及许多蓝藻产生，但它们在这些光合作用生物中的功能仍不完全清楚。我们以前曾报道，拟南芥维生素E缺乏2(Vte2)突变体对低温(LT)敏感，这是由于韧皮部传递细胞中大量老茧蛋白沉积导致传递细胞壁(TCW)发育受阻和光同化产物输出受到影响。为了进一步了解生育酚在LT诱导的TCW发育中的作用，我们比较了LT处理过程中vte2和野生型叶片的整体转录谱。在允许的条件下，生育酚缺乏对整体基因表达没有显著影响，但在LT处理48h后显著影响77个基因的表达。与野生型相比，在vte2中，与溶质运输相关的基因被抑制，而参与病原菌反应和细胞壁修饰的基因被诱导，包括类葡聚糖合成酶4(GSL4)和GSL11这两个基因家族的成员。然而，在vte2背景中单独引入gsl4或gsl11突变并不能抑制痂沉积或LT诱导的vte2的整体表型。耐人寻味的是，将破坏GSL5的突变引入VTE2，大大减少了LT的维管痂沉积，但同样对LT处理的VTE2的光同化物输出表型没有影响。这些结果表明，在经LT处理的VTE2中，GSL5在TCW中起主要作用，但这种依赖于GSL5的痂沉积并不是光合作用同化物输出表型受损的主要原因。
Tocopherols (vitamin E) are lipid-soluble antioxidants produced by all plants and algae, and many cyanobacteria, yet their functions in these photosynthetic organisms are still not fully understood. We have previously reported that the vitamin E deficient 2 (vte2) mutant of Arabidopsis thaliana is sensitive to low temperature (LT) due to impaired transfer cell wall (TCW) development and photoassimilate export associated with massive callose deposition in transfer cells of the phloem. To further understand the roles of tocopherols in LT induced TCW development we compared the global transcript profiles of vte2 and wild-type leaves during LT treatment. Tocopherol deficiency had no significant impact on global gene expression in permissive conditions, but significantly affected expression of 77 genes after 48 h of LT treatment. In vte2 relative to wild type, genes associated with solute transport were repressed, while those involved in various pathogen responses and cell wall modifications, including two members of callose synthase gene family, GLUCAN SYNTHASE LIKE 4 (GSL4) and GSL11, were induced. However, introduction of gsl4 or gsl11 mutations individually into the vte2 background did not suppress callose deposition or the overall LT-induced phenotypes of vte2. Intriguingly, introduction of a mutation disrupting GSL5, the major GSL responsible for pathogen-induced callose deposition, into vte2 substantially reduced vascular callose deposition at LT, but again had no effect on the photoassimilate export phenotype of LT-treated vte2. These results suggest that GSL5 plays a major role in TCW callose deposition in LT-treated vte2 but that this GSL5-dependent callose deposition is not the primary cause of the impaired photoassimilate export phenotype.
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影响因子：
3.5
作者：
Hohnjec, Natalija;Lenz, Frauke;Kuester, Helge
通讯作者：
Kuester, Helge
影响因子：
4.6
作者：
Guseman, Jessica M.;Lee, Jin Suk;Torii, Keiko U.
通讯作者：
Torii, Keiko U.
影响因子：
11.6
作者：
Hong, ZL;Delauney, AJ;Verma, DPS
通讯作者：
Verma, DPS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.4
作者：
Gohil, K;Schock, BC;Traber, MG
通讯作者：
Traber, MG