Isolation and characterization of a dnaK genomic locus in a halotolerant cyanobacterium Aphanothece halophytica
Isolation and characterization of a dnaK genomic locus in a halotolerant cyanobacterium Aphanothece halophytica
复制标题
耐盐蓝藻 Aphanothece halophytica 中 dnaK 基因组位点的分离和表征
作者：
Byung;T. Hibino;J. Jo;A. Viale;T. Takabe
我们克隆和表征的基因组位点编码的DnaK/热休克蛋白70家族的分子伴侣，dnaK 1，从耐盐蓝藻Aphanothece盐生。在大肠杆菌中与植物质体蓝素前体共表达dnaK 1导致植物蛋白的溶解度急剧增加。这表明A.盐生植物dnaK 1编码具有DnaK/Hsp 70分子伴侣成员功能的功能蛋白。助理盐生植物dnaK 1基因座还包括grpE-dnaK 1-dnaJ顺序的grpE和dnaJ同源基因。蓝藻细胞受到热胁迫后，dnaK 1的转录本含量显著增加。利用特异性探针进行的北方分析表明，2.8、2.2、1.3和0.7 kb的转录物种类分别包括grpE-dnaK 1、dnaK 1、dnaJ和grpE。这表明在该基因座中存在不同的终止子和/或热应激启动子。在高渗环境中，蓝藻细胞中dnaK 1的转录和蛋白水平均增加，提示DnaK 1在保护和/或恢复A.盐生植物从这种特殊的压力。
We cloned and characterized a genomic locus encoding a distinct member of the DnaK/Hsp70 family of molecular chaperones, dnaK1, from the halotolerant cyanobacterium Aphanothece halophytica. Co-expression of dnaK1 with a plant plastocyanin precursor in Escherichia coli resulted in a dramatic increase in the solubility of the plant protein. This indicates that A. halophytica dnaK1 encodes a functional protein possessing functions assigned to DnaK/Hsp70 chaperone members. The A. halophytica dnaK1 locus also encompasses grpE and dnaJ homologue genes in the order grpE-dnaK1-dnaJ. The transcript content of dnaK1 increased strongly upon subjecting cyanobacterial cells to heat stress. Northern analyses using specific probes indicated transcript species of 2.8, 2.2, 1.3, and 0.7 kb, which comprised grpE-dnaK1, dnaK1, dnaJ, and grpE, respectively. This indicates the presence of different terminators and/or heat stress promoters in this locus. Both dnaK1 transcript and protein levels increased in cyanobacterial cells transferred to hyperosmotic environments, suggesting a role of DnaK1 in the protection and/or recovery of A. halophytica from this particular stress.
影响因子：
2.8
作者：
Caslake, LF;Bryant, DA
通讯作者：
Bryant, DA
一种西格玛因子，可改变蓝细菌中部分基因的昼夜节律表达。
DOI：
--
发表时间：
1996
期刊：
The EMBO journal
影响因子：
--
作者：
Tsinoremas,NF;Ishiura,M;Kondo,T;Andersson,CR;Tanaka,K;Takahashi,H;Johnson,CH;Golden,SS
通讯作者：
Golden,SS