Antiprothrombin antibodies-are they worth assaying?
Antiprothrombin antibodies-are they worth assaying?
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DOI：
10.1016/j.thromres.2004.08.024
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发表时间：
2004-01-01
影响因子：
7.5
通讯作者：
Koike, T
中科院分区：
文献类型：
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作者：
Atsumi, T;Amengual, O;Koike, T
根据抗磷脂综合征（APS）的初步分类标准（札幌标准），β（2）-糖蛋白I（β（2）GPI）依赖性抗心磷脂抗体（aCL）和狼疮抗凝剂（LA）是被认为是APS分类标准的唯一实验室检查。最近，已检测到抗磷脂酰丝氨酸-凝血酶原复合物（aPS/PT）的抗体，这些抗体，而不是单独抗凝血酶原的抗体，与APS和LA密切相关。我们评估了aPS/PT在我们的各种自身免疫性疾病患者人群中诊断APS的敏感性和特异性，并研究了aPS/PT是否可用作疑似APS患者的诊断试验。研究人群包括219例自身免疫性疾病患者，包括82例APS患者和137例无APS患者（55例系统性红斑狼疮，32例类风湿性关节炎，10例原发性干燥综合征，8例硬皮病，5例白塞氏病和27例其他风湿性疾病）。在CaCl 2存在下，使用磷脂酰丝氨酸-凝血酶原复合物作为固定在ELISA板上的抗原，通过ELISA测量IgG/M aPS/PT。通过标准方法测量IgG/M aCL，并通过凝血测定法检测LA。有APS的患者中aPS/PT、aCL和LA的发生率（分别为47、46和69）高于无APS的患者（分别为11、19和29）（OR 95% [CI];分别为15.4 [7.2-32.7]、7.9 [4.1-15.2，19.8 [9.6-40.6]）。每种检测方法诊断APS的敏感性分别为57%、56%和86%，特异性分别为92%、86%和79%。aPS/PT与aCL对APS的诊断价值相似，因此，aPS/PT不仅可用于研究，而且可作为APS的实验室分级标准之一。(c)2004年由Elsevier Ltd.出版。
According to the preliminary classification criteria of the antiphospholipid syndrome (APS) (Sapporo Criteria), beta(2)-glycoprotein I (beta(2)GPI)-dependent anticardiolipin antibodies (aCL) and Lupus anticoagulant (LA) are the only laboratory tests considered as criteria for the classification of the APS. Recently, antibodies against phosphatidylserine-prothrombin complex (aPS/PT) have been detected and these antibodies, rather than antibodies against prothrombin alone, are closely associated with APS and LA. We assessed the sensitivity and specificity of aPS/PT for the diagnosis of APS in our population of patients with a variety of autoimmune disorders and investigated whether aPS/PT could be used as diagnostic test in patients suspected of having APS. The study population comprised 219 patients with autoimmune diseases including 82 patients with APS and 137 without APS (55 systemic lupus erythematosus, 32 rheumatoid arthritis, 10 primary Sjogren's syndrome, 8 scleroderma, 5 Behcet's disease and 27 other rheumatic diseases). IgG/M aPS/PT were measured by ELISA using phosphatidylserine-prothrombin complex as antigen immobilized on ELISA plates in the presence of CaCl2. IgG/M aCL were measured by standard methods and LA was detected by clotting assays. aPS/PT, aCL and LA were more frequently found in patients with APS (47, 46 and 69, respectively) than in those without APS (11, 19 and 29, respectively) (OR 95% [CI]; 15.4 [7.2-32.7], 7.9 [4.1-15.2, 19.8 [9.6-40.6], respectively]. The sensitivity of each assay for the diagnosis of APS was 57%, 56% and 86% with a specificity of 92%, 86% and 79%, respectively. aPS/PT and aCL have similar diagnostic value for APS, therefore, we propose that aPS/PT should be further explored, not only for research purposes, but also as a candidate of one of the laboratory criteria for the classification of the APS. (c) 2004 Published by Elsevier Ltd.