Isolation and characterization of microsatellite markers in Dendrobium officinale, an endangered herb endemic to China
Isolation and characterization of microsatellite markers in Dendrobium officinale, an endangered herb endemic to China
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DOI：
10.1111/j.1471-8286.2007.01817.x
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发表时间：
2007-11
期刊：
影响因子：
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通讯作者：
S. Gu;Xiaoyu Ding;Y. Wang;Qi Zhou;G. Ding;Xuexia X. Li;Liang Qian
中科院分区：
文献类型：
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作者：
S. Gu;Xiaoyu Ding;Y. Wang;Qi Zhou;G. Ding;Xuexia X. Li;Liang Qian
铁皮石斛是一种珍贵的草本植物，自1950年以来，由于人类的开发和生境的恶化，铁皮石斛面临着严重的危险。为了研究该物种的遗传多样性和结构，我们从两个微卫星富集文库中分离并鉴定了12个微卫星位点。对22例Leye种群个体进行了分析。这些位点具有多态性，每个位点显示3 ~ 12个等位基因。观察到的杂合度为0.150 ~ 0.624，期望杂合度为0.162 ~ 0.605。这些微卫星位点的发现，将有助于对其保护、遗传多样性、种群结构和个体鉴定等方面的研究。
As an invaluable herb, Dendrobium officinale has been in severe danger since 1950 because of human exploitation and habitat deterioration. In order to investigate the genetic diversity and structure of this species, we isolated and characterized 12 microsatellite loci derived from two microsatellite-enriched libraries. Twenty-two individuals from Leye population were analysed. These loci were polymorphic and displayed three to 12 alleles per locus. The observed and expected heterozygosities ranged from 0.150 to 0.624 and from 0.162 to 0.605, respectively. These are the first microsatellite loci characterized from D. officinale that will contribute to research on the conservation, genetic diversity, population structure and individual authentication.