Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation.
Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation.
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亚硝酸盐抑制血小板取决于红细胞和脱氧。
DOI：
10.1371/journal.pone.0030380
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sibmooh N
中科院分区：
文献类型：
--
作者：
Srihirun S;Sriwantana T;Unchern S;Kittikool D;Noulsri E;Pattanapanyasat K;Fucharoen S;Piknova B;Schechter AN;Sibmooh N
亚硝酸盐是组织和血液中的一氧化氮（NO）代谢产物，在缺氧条件下可转化为NO，促进组织灌注。虽然已知亚硝酸盐还原为NO后会引起血管舒张，但亚硝酸盐对血小板活性的影响仍不清楚。在这项研究中，亚硝酸盐和亚硝酸盐+红细胞，脱氧和不脱氧，对血小板活性的影响进行了研究。分别通过比浊法和阻抗聚集法研究了富血小板血浆（PRP）和PRP+红细胞中的血小板聚集。在PRP中，DEANONOate抑制ADP诱导的血小板聚集，而亚硝酸盐对血小板无影响。在PRP+红细胞中，DEANONOate对血小板的抑制作用降低，而生理浓度（0.1 µM）的亚硝酸盐抑制血小板聚集和ATP释放。亚硝酸盐+红细胞对血小板的作用被C-PTIO（一种膜不可渗透的NO清除剂）消除，表明NO介导的作用。此外，脱氧增强亚硝酸盐的效果，从血小板中P-选择素表达的减少和cGMP水平的增加观察。ADP诱导的全血血小板聚集与全血和红细胞亚硝酸盐水平呈负相关。生理水平的亚硝酸盐单独对血浆中的血小板没有影响。亚硝酸盐在红细胞存在下通过还原为NO抑制血小板，这是由脱氧促进的。亚硝酸盐可能在调节循环中的血小板活性中起作用，特别是在缺氧期间。
Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated. Platelet aggregation was studied in platelet-rich plasma (PRP) and PRP+erythrocytes by turbidimetric and impedance aggregometry, respectively. In PRP, DEANONOate inhibited platelet aggregation induced by ADP while nitrite had no effect on platelets. In PRP+erythrocytes, the inhibitory effect of DEANONOate on platelets decreased whereas nitrite at physiologic concentration (0.1 µM) inhibited platelet aggregation and ATP release. The effect of nitrite+erythrocytes on platelets was abrogated by C-PTIO (a membrane-impermeable NO scavenger), suggesting an NO-mediated action. Furthermore, deoxygenation enhanced the effect of nitrite as observed from a decrease of P-selectin expression and increase of the cGMP levels in platelets. The ADP-induced platelet aggregation in whole blood showed inverse correlations with the nitrite levels in whole blood and erythrocytes. Nitrite alone at physiological levels has no effect on platelets in plasma. Nitrite in the presence of erythrocytes inhibits platelets through its reduction to NO, which is promoted by deoxygenation. Nitrite may have role in modulating platelet activity in the circulation, especially during hypoxia.
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