Molecular Epidemiology of Rotavirus Gastroenteritis in Central Kenya Before Vaccine Introduction
Molecular Epidemiology of Rotavirus Gastroenteritis in Central Kenya Before Vaccine Introduction
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疫苗引入前肯尼亚中部轮状病毒胃肠炎的分子流行病学
DOI：
10.1002/jmv.24691
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发表时间：
2016
影响因子：
12.7
通讯作者：
Koki Taniguchi,* and Yoshio Ichinose
中科院分区：
文献类型：
--
作者：
Ernest A. Wandera;Shah Mohammad;Satoshi Komoto;Yoshimasa Maeno;James Nyangao;Tomihiko Ide;Cyrus Kathiiko;Erick Odoyo;Takao Tsuji;Koki Taniguchi,* and Yoshio Ichinose
2009年7月至2014年6月期间，共采集了肯尼亚中部Kiambu县医院收治的5岁以下患有急性胃肠炎的儿童的粪便样本1,546份。使用ELISA对标本进行A组轮状病毒（RVA）筛查，并对RVA阳性标本进行半巢式RT-PCR以确定G和P基因型。在 429/1,546 (27.5%) 粪便样本中检测到 RVA。 RVA 感染发生在 59 个月以下的所有年龄组中，早期高峰出现在 6-17 个月大。感染全年持续存在，不同年份有明显的季节性高峰。 G1P[8] (28%) 是最主要的基因型，其次是 G9P[8] (12%)、G8P[4] (7%)、G1P[4] (5%)、G9P[4] (4%) 和 G12P[6] (3%)。在G和P基因型的年度变化中，2012年发现了从G9P[8]到G1P[8]的重大转变。对具有异常G8或P[6]的7个品系的VP7和VP4基因核苷酸序列进行系统发育分析表明，G8的VP7核苷酸序列聚集在包含非洲品系的谱系6中，并且至少有两个不同的VP4核苷酸序列P[6]菌株。这些结果代表了疫苗引入前该地区流行的 RVA 毒株的基本数据。医学。病毒。 89:809–817，2017 年。© 2016 Wiley periodicals, Inc.
Between July 2009 and June 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to Kiambu County Hospital, Central Kenya. The specimens were screened for group A rotavirus (RVA) using ELISA, and RVA‐positive specimens were subjected to semi‐nested RT‐PCR to determine the G and P genotypes. RVA was detected in 429/1,546 (27.5%) fecal specimens. RVA infections occurred in all age groups <59 months, with an early peak at 6–17 months. The infections persisted year‐round with distinct seasonal peaks depending on the year. G1P[8] (28%) was the most predominant genotype, followed by G9P[8] (12%), G8P[4] (7%), G1P[4] (5%), G9P[4] (4%), and G12P[6] (3%). In the yearly change of G and P genotypes, a major shift from G9P[8] to G1P[8] was found in 2012. Phylogenetic analysis of the nucleotide sequences of the VP7 and VP4 genes of seven strains with unusual G8 or P[6] showed that the VP7 nucleotide sequences of G8 were clustered in lineage 6 in which African strains are included, and that there are at least two distinct VP4 nucleotide sequences of P[6] strains. These results represent basic data on RVA strains circulating in this region before vaccine introduction.J. Med. Virol. 89:809–817, 2017. © 2016 Wiley Periodicals, Inc.