Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome (Retracted article. See vol. 334, pg. 1636, 2011)
Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue Syndrome (Retracted article. See vol. 334, pg. 1636, 2011)
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DOI：
10.1126/science.1179052
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发表时间：
2009-10-23
期刊：
影响因子：
56.9
通讯作者：
Mikovits, Judy A.
中科院分区：
文献类型：
--
作者：
Lombardi, Vincent C.;Ruscetti, Francis W.;Mikovits, Judy A.
慢性疲劳综合征（CFS）是一种病因不明的使人衰弱的疾病，估计影响全球1700万人。通过研究CFS患者的外周血单个核细胞（PBMC），我们在101例患者中的68例（67%）和218例健康对照中的8例（3.7%）中鉴定了来自人类γ逆转录病毒（异嗜性小鼠白血病病毒相关病毒（XMRV））的DNA。细胞培养实验表明，患者来源的XMRV是感染性的，并且病毒的细胞相关和无细胞传播都是可能的。在未感染的原代淋巴细胞和指示细胞系暴露于活化的PBMC、B细胞、T细胞或来源于CFS患者的血浆后，在未感染的原代淋巴细胞和指示细胞系中建立继发性病毒感染。这些发现提出了XMRV可能是CFS发病机制中的一个促成因素的可能性。
Chronic fatigue syndrome (CFS) is a debilitating disease of unknown etiology that is estimated to affect 17 million people worldwide. Studying peripheral blood mononuclear cells (PBMCs) from CFS patients, we identified DNA from a human gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), in 68 of 101 patients (67%) as compared to 8 of 218 (3.7%) healthy controls. Cell culture experiments revealed that patient-derived XMRV is infectious and that both cell-associated and cell-free transmission of the virus are possible. Secondary viral infections were established in uninfected primary lymphocytes and indicator cell lines after their exposure to activated PBMCs, B cells, T cells, or plasma derived from CFS patients. These findings raise the possibility that XMRV may be a contributing factor in the pathogenesis of CFS.