ADHESION RECEPTOR PHENOTYPES OF MURINE LUNG CD4(+) T-CELLS DURING THE PULMONARY IMMUNE-RESPONSE TO SHEEP ERYTHROCYTES
ADHESION RECEPTOR PHENOTYPES OF MURINE LUNG CD4(+) T-CELLS DURING THE PULMONARY IMMUNE-RESPONSE TO SHEEP ERYTHROCYTES
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DOI：
10.1165/ajrcmb.12.5.7537969
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发表时间：
1995-05-01
影响因子：
6.4
通讯作者：
BUECHNERMAXWELL, VA
中科院分区：
文献类型：
--
作者：
CURTIS, JL;KIM, S;BUECHNERMAXWELL, VA
了解肺淋巴细胞募集的分子机制是选择性控制免疫功能低下宿主的免疫性肺部疾病和感染的关键一步。为了剖析这些机制，我们正在研究在致敏的C57 BL/6小鼠中通过用T细胞依赖性抗原绵羊红细胞（SRBC）进行肠道内攻击诱导的应答。本研究使用四参数流式细胞术检测外周血和肺中CD 4（+）小鼠T细胞对已知在致敏人淋巴细胞上差异表达的受体（CD 2、CD 11 a、CD 44、CD 45 RB、CD 49 d和L-选择素）的表达。与外周血相比，通过支气管肺泡灌洗（BAL）回收的肺CD 4（+）T细胞更多地表现出致敏表型。以CD 45 RB和L-selectin的低表达判断，BAL中76 ~ 90%的CD 4（+）T细胞被致敏。BAL和肺间质中CD 4（+）T细胞的粘附受体表型基本一致，但致敏细胞的比例较高。高表达CD 44和CD 49 d的CD 4（+）T细胞百分比在应答后期增加。然而，当仅考虑可能介导募集的上调粘附受体时，BAL中22 - 52%的CD 4（+）T细胞没有增加粘附受体表达。引发和激发之间的较长持续时间并没有增加粘附受体上调。高粘附受体的表达是最明显的最大淋巴细胞流入期间，这表明器官非特异性粘附受体的表面密度增加以外的因素有助于淋巴细胞募集肺免疫反应。
Understanding the molecular mechanisms of pulmonary lymphocyte recruitment is a crucial step toward selective control of immune lung diseases and infections in immunocompromised hosts. To dissect these mechanisms, we are studying the response induced in primed C57BL/6 mice by intratracheal challenge with the T cell-dependent antigen, sheep red blood cells (SRBC). This study used four-parameter flow cytometry to examine expression by CD4(+) murine T cells in peripheral blood and lungs of receptors known to be differentially expressed on primed human lymphocytes (CD2, CD11a, CD44, CD45RB, CD49d, and L-selectin). Compared with peripheral blood, more lung CD4(+) T cells recovered by bronchoalveolar lavage (BAL) showed a primed phenotype. Judged by low expression of CD45RB or L-selectin, 76 to 90% of BAL CD4(+) T cells were primed at all times, Adhesion receptor phenotype of CD4(+) T cells in BAL and lung interstitium agreed closely, although BAL contained a greater percentage of primed cells. The percentage of CD4(+) T cells with high expression of CD44 and CD49d increased late in the response. However, when considering only upregulated adhesion receptors which might mediate recruitment, 22 to 52% of CD4(+) T cells in BAL did not have increased adhesion receptor expression. Longer duration between priming and challenge did not increase adhesion receptor upregulation. High adhesion receptor expression was least evident during the periods of maximal lymphocyte influx, suggesting that factors other than increased surface density of organ-nonspecific adhesion receptors contribute to lymphocyte recruitment during pulmonary immune responses.