Structure/epitope-based immunoinformatics analysis of structural proteins of 2019 novel coronavirus
Structure/epitope-based immunoinformatics analysis of structural proteins of 2019 novel coronavirus
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DOI：
10.21203/rs.3.rs-50740/v1
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
Yuwei Li;M. Mao;Liteng Yang;Xizhuo Sun;N. Zhong;Zhi-gang Liu
中科院分区：
文献类型：
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作者：
Yuwei Li;M. Mao;Liteng Yang;Xizhuo Sun;N. Zhong;Zhi-gang Liu
新发现的2019年新型冠状病毒（2019- ncov）已造成81400多例实验室确诊的人类感染，其中3261例死亡，对人类健康构成严重威胁。然而，目前还没有专门的抗病毒治疗方法或疫苗。为鉴定抗新型冠状病毒感染全球肽疫苗所需的免疫优势肽，采用生物信息学方法分析了新型冠状病毒结构蛋白的结构和免疫原性。通过不同的免疫信息学工具分别测定了4种结构蛋白中的33个b细胞表位和39个t细胞表位，其中包括刺突蛋白（22个b细胞表位，25个t细胞表位）、核衣壳蛋白（7个b细胞表位，6个t细胞表位）、膜蛋白（2个b细胞表位，7个t细胞表位）和包膜蛋白（2个b细胞表位，1个t细胞表位）。用一级序列表位残基的比例来测定蛋白质的抗原性和免疫原性。包膜蛋白具有最大的抗原性，其b细胞表位的残基覆盖率为24%。膜蛋白具有最大的免疫原性，其t细胞表位的残基覆盖率为55.86%。新型冠状病毒引起免疫风暴的原因可能是感染细胞中膜和包膜蛋白的大量表达。此外，有必要进行实验验证这些预测表位的研究，以确保b细胞和t细胞刺激的潜力，使其有效地用作候选疫苗。这些发现为进一步研究该病的发病机制，优化该病的诊断、抗病毒和疫苗接种策略提供了基础。
The newly identified 2019 novel coronavirus (2019-nCoV) has caused more than 81,400 laboratory-confirmed human infections, including 3261 deaths, posing a serious threat to human health. Currently, however, there is no specific antiviral treatment or vaccine. To identify immunodominant peptides for designing global peptide vaccine for combating the infections caused by 2019-nCoV, the structure and immunogenicity of 2019-nCoV structural protein were analyzed by bioinformatics tools. 33 B-cell epitopes and 39 T-cell epitopes were determined in four structural proteins via different immunoinformatic tools in which include spike protein (22 B-cell epitopes, 25 T-cell epitopes ), nucleocapsid protein (7 B-cell epitopes, 6 T-cell epitopes), membrane protein (2 B-cell epitopes, 7 T-cell epitopes), and envelope protein (2 B-cell epitopes, 1T-cell epitopes), respectively. The proportion of epitope residues in primary sequence was used to determine the antigenicity and immunogenicity of proteins. The envelope protein has the largest antigenicity in which residue coverage of B-cell epitopes is 24%. The membrane protein possesses the largest immunogenicity in which residue coverage of T-cell epitopes is 55.86%. The reason that immune storm was caused by 2019-nCoV maybe that the membrane and envelope protein expressed plentifully in cell infected. Further, studies involving experimental validation of these predicted epitopes is warranted to ensure the potential of B-cells and T-cells stimulation for their effective use as vaccine candidates. These findings provide the basis for starting further studies on the pathogenesis, and optimizing the design of diagnostic, antiviral and vaccination strategies for this emerging infection.