Measles virus-induced disruption of the glial-fibrillary-acidic protein cytoskeleton in an astrocytoma cell line (U-251)
Measles virus-induced disruption of the glial-fibrillary-acidic protein cytoskeleton in an astrocytoma cell line (U-251)
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DOI：
10.1128/jvi.74.8.3874-3880.2000
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发表时间：
2000-04-01
影响因子：
5.4
通讯作者：
Rima, BK
中科院分区：
文献类型：
--
作者：
Duprex, WP;McQuaid, S;Rima, BK
用表达增强型绿色荧光蛋白（MVeGFP）的重组麻疹病毒感染2株星形细胞瘤细胞株GCCM和U-251，研究病毒感染对细胞骨架的影响。间接免疫细胞化学用于证明细胞骨架成分的细胞定位。采用增强绿色荧光蛋白自身荧光法对麻疹病毒感染细胞进行鉴定。在两种细胞类型中均未观察到细胞骨架的肌动蛋白、微管蛋白或波形蛋白成分的改变，而在mvegfp感染的U-251细胞中，胶质-纤维-酸性蛋白丝（GFAP）网络被破坏。通过对共聚焦显微镜获得的数据集进行图像分析，定量感染和未感染U-251细胞中GFAP的相对含量，使用vimentin（另一种对MVeGFP没有影响的中间丝）作为对照。
A recombinant measles virus which expresses enhanced green fluorescent protein (MVeGFP) has been used to infect two astrocytoma cell lines (GCCM and U-251) to study the effect of virus infection on the cytoskeleton. Indirect immunocytochemistry was used to demonstrate the cellular localization of the cytoskeletal components. Enhanced green fluorescent protein autofluorescence was used to identify measles virus-infected cells. No alteration of the actin, tubulin, or vimentin components of the cytoskeleton was observed in either cell type, whereas a disruption of the glial-fibrillary-acidic protein filament (GFAP) network was noted in MVeGFP-infected U-251 cells. The relative amounts of GFAP present in infected and uninfected U-251 cells were quantified by image analysis of data sets obtained by confocal microscopy by using vimentin, another intermediate filament on which MVeGFP has no effect, as a control.