Nuclear Bodies Built on Architectural Long Noncoding RNAs: Unifying Principles of Their Construction and Function.
Nuclear Bodies Built on Architectural Long Noncoding RNAs: Unifying Principles of Their Construction and Function.
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DOI：
10.14348/molcells.2017.0263
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发表时间：
2017-12-31
影响因子：
3.8
通讯作者：
Hirose T
中科院分区：
文献类型：
--
作者：
Chujo T;Hirose T
核体是浓缩特定蛋白质和/或RNA的亚核、球形和无膜区室。它们作为特定RNA、蛋白质或核糖核蛋白复合物的生物发生、储存和隔离的位点。最近的研究表明，在各种真核生物的核体的子集是由建筑长非编码RNA（arcRNA）。在这里，我们描述了这些机构的建设和功能的统一的机械原理，特别是专注于液-液相分离诱导的建筑分子，形成多个弱粘合剂的相互作用。我们还讨论了使用arcRNA而不是建筑蛋白质来构建身体的三个可能的优点：位置特异性，快速性和经济性。此外，我们介绍了两个最近设计的方法来发现新的arcRNA构建的机构，一个专注于这些机构的RNA酶敏感性，另一个专注于“半提取”的arcRNA。
Nuclear bodies are subnuclear, spheroidal, and membraneless compartments that concentrate specific proteins and/or RNAs. They serve as sites of biogenesis, storage, and sequestration of specific RNAs, proteins, or ribonucleoprotein complexes. Recent studies reveal that a subset of nuclear bodies in various eukaryotic organisms is constructed using architectural long noncoding RNAs (arcRNAs). Here, we describe the unifying mechanistic principles of the construction and function of these bodies, especially focusing on liquid-liquid phase separation induced by architectural molecules that form multiple weakly adhesive interactions. We also discuss three possible advantages of using arcRNAs rather than architectural proteins to build the bodies: position-specificity, rapidity, and economy in sequestering nucleic acid-binding proteins. Moreover, we introduce two recently devised methods to discover novel arcRNA-constructed bodies; one that focuses on the RNase-sensitivity of these bodies, and another that focuses on “semi-extractability” of arcRNAs.