Amycolatopsis pithecelloba sp. nov., a novel actinomycete isolated from roots of Pithecellobium dulce in Thailand
Amycolatopsis pithecelloba sp. nov., a novel actinomycete isolated from roots of Pithecellobium dulce in Thailand
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DOI：
10.1038/s41429-019-0271-z
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Ratchanee Mingma;Yuki Inahashi;A. Matsumoto;Yōko Takahashi;Kannika Duangmal
中科院分区：
文献类型：
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作者：
Ratchanee Mingma;Yuki Inahashi;A. Matsumoto;Yōko Takahashi;Kannika Duangmal
采用多相分类学方法对从泰国杜尔黄树根中分离的放线菌菌株 RM579T 进行了研究。 16S rRNA基因序列比对和系统发育分析结果表明，菌株RM579T属于假诺卡氏菌科，与Amycolatopsis属（序列相似性≤94.5%）和Haloechinothrix属（序列相似性≤93.4%）成员亲缘关系最近。通过邻接法构建的系统发育树表明，菌株RM579T位于Amycolatopsis属的进化枝内，与Amycolatopsis taiwanensis0345M-7T、Amycolatopsis colonyataTT00-43T和Amycolatopsis helveolaTT99-32T形成单系簇。菌株RM579T形成白色气生菌丝体和黄棕色基质菌丝体，其碎裂成杆状元件。 RM579T菌株的形态特征和化学分类学特征与Amycolatopsis属一致。 RM579T菌株的细胞壁含有内消旋二氨基庚二酸。阿拉伯糖、半乳糖、甘露糖和核糖被检测为全细胞糖。不存在霉菌酸。细胞壁中胞壁酸的酰基类型为N-乙酰基。检测到二磷脂酰甘油、羟基磷脂酰乙醇胺、磷脂酰肌醇和未鉴定的磷脂作为极性脂质。主要细胞脂肪酸（>10%）是 iso-C16:0、iso-C16:02-OH 和 C16:1cis9。由此产生的数据表明菌株RM579T应被归类为Amycolatopsis属新物种的代表，其名称为Amycolatopsis Pithecellobasp。十一月被提议。模式菌株为 RM579T(=TBRC 1849T=NBRC 106096T)。
An actinomycete strain RM579Tisolated from roots ofPithecellobium dulcein Thailand was studied using the polyphasic taxonomic approach. The results of comparative 16S rRNA gene sequence and phylogenetic analysis showed that strain RM579Tbelonged to the familyPseudonocardiaceaeand it was most closely related to members of the generaAmycolatopsis(≤94.5% sequence similarity) andHaloechinothrix(≤93.4% sequence similarity). A phylogenetic tree constructed by neighbor-joining method indicated that strain RM579Twas positioned within the clade of the genusAmycolatopsisand formed a monophyletic cluster withAmycolatopsis taiwanensis0345M-7T,Amycolatopsis pigmentataTT00-43TandAmycolatopsis helveolaTT99-32T. Strain RM579Tformed white aerial mycelium and yellowish-brown substrate mycelium that fragments into rod-shaped elements. Morphological features and chemotaxonomic characteristics of strain RM579Twere consistent with those of the genusAmycolatopsis. The cell wall of strain RM579Tcontainedmeso-diaminopimelic acid. Arabinose, galactose, mannose, and ribose were detected as whole-cell sugars. Mycolic acids were absent. The acyl type of the muramic acid in the cell wall was N-acetyl. Diphosphatidylglycerol, hydroxyl-phosphatidylethanolamine, phosphatidylinositol, and an unidentified phospholipid were detected as polar lipids. The major cellular fatty acids (>10%) were iso-C16:0, iso-C16:02-OH, and C16:1cis9. The resultant data indicated that strain RM579Tshould be classified as representative of a novel species in the genusAmycolatopsis, for which the nameAmycolatopsis pithecellobasp. nov. is proposed. The type strain is RM579T(=TBRC 1849T=NBRC 106096T).