Human CD3+CD56+NKT-like cells express a range of complement receptors and C3 activation has negative effects on these cell activity and effector function
Human CD3+CD56+NKT-like cells express a range of complement receptors and C3 activation has negative effects on these cell activity and effector function
复制标题
人 CD3( )CD56( )NKT 样细胞表达一系列补体受体，C3 激活对这些细胞活性和效应器功能产生负面影响
DOI：
10.1016/j.humimm.2021.06.001
复制
发表时间：
2021-08-13
期刊：
影响因子：
2.7
通讯作者：
Li, Ke
中科院分区：
文献类型：
--
作者：
Min, Xiao-Yun;Liu, Cheng-Fei;Li, Ke
CD3(+)CD56(+)NKT样细胞是一种罕见的淋巴细胞群，在各种类型的免疫相关疾病，特别是癌症中发挥重要作用。补体系统通过与一系列免疫细胞上表达的补体受体相互作用来调节炎症和免疫反应。然而，CD3(+)CD56(+)NKT样细胞是否受补体系统调节尚未明确。在本研究中，使用PCR和流式细胞术评估了从人外周血中分离的门控CD3(+)CD56(+)NKT样细胞中补体受体和调节因子的表达。结果显示，人CD3(+)CD56(+)NKT样细胞表达一系列补体受体和调节因子，如CR3、C3aR、C5aR、C5L2、CD46和CD55。此外，补体成分3 (C3)（培养物上清液中补体激活的关键成分）的存在减轻了CD3(+)CD56(+)NKT样细胞的活性、IFN-γ产生和杀伤功能。本研究提供了支持补体激活与CD3(+)CD56(+)NKT样细胞功能调节之间关系的证据，扩展了我们对补体调节网络的了解，并强调了治疗多种免疫相关疾病的潜在靶标，特别是基于NKT细胞的肿瘤过继免疫治疗。 (C) 2021 年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
CD3(+)CD56(+)NKT-like cells are a rare population of lymphocytes that serve important roles in various types of immune-related diseases, and particularly in cancer. The complement system regulates inflammatory and immune responses by interacting with complement receptors expressed on a range of immune cells. However, whether CD3(+)CD56(+)NKT-like cells are regulated by the complement system has still not been definitively determined. In the present study, the expression of complement receptors and regulators in gated CD3(+)CD56(+)NKT-like cells isolated from human peripheral blood was assessed using PCR and flow cytometry. The results showed that human CD3(+)CD56(+)NKT-like cells expressed a range of complement receptors and regulators, such as CR3, C3aR, C5aR, C5L2, CD46 and CD55. Furthermore, the presence of complement component 3 (C3), a key component in complement activation in culture supernatant, mitigated the activity, IFN-gamma production and killing function of CD3(+)CD56(+)NKT-like cells. The present study provides evidences supporting the relationship between complement activation and functional modulation of CD3(+)CD56(+)NKT-like cells, expanding our knowledge of the complement regulatory network, and also highlighting a potential target for treatment of numerous immune-related diseases, particularly NKT cell-based tumor adoptive immunotherapy. (C) 2021 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.